慢性胰腺炎大鼠肝组织NF-κB的表达及中药的干预作用  

Expression of NF-κB in Hepatic Cell of Chronic Pancreatitis Rats Model and Intervening Effect of Traditional Medicine

作　　者：张鹏[1] 赵战朝[1] 梁泉[1] 薛承锐[1] 

机构地区：[1]天津医科大学总医院中西医结合外科,天津300052

出　　处：《中国中西医结合外科杂志》2008年第5期490-493,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的:研究实验性慢性胰腺炎大鼠肝组织核转录因子(NF-κB)的表达以及药物的干预作用。方法:建立慢性胰腺炎大鼠模型,Western印迹法检测各组大鼠肝组织NF-κB蛋白的表达,应用放免法检测血清IL-6、TNF-α的水平。结果:慢性胰腺炎模型组大鼠肝组织NF-κB蛋白的表达较对照组明显升高(P<0.05),且血清细胞因子IL-6、TNF-α含量增加。大承气颗粒治疗组及丹参治疗组NF-κB的表达较模型组明显降低(P<0.05)。结论:慢性胰腺炎导致大鼠肝组织NF-κB蛋白的表达升高,大承气颗粒及丹参对NF-κB蛋白的表达有明显的抑制作用。Objective To explore the expression of nuclear factor - κB （ NF - κB） in hepatocytes of chronic pancreatitis and the role of herbal medication. Methods Chronic pancreatitis rat model was established by injection of oleic acid into the rat pancreatic duct. Six weeks later hepatic NF- κB activation was determined by Western blot method and immunohistochemistry method. The cytokines were measured with radioimmunoassay. Results Compared with the sham operation group, the NF- κB activation level of the model group was increased significantly. While compared with the chronic pancreatitis model group, the NF -κB activation level of medication group was decreased. Conclusion Chronic pancreatitis causes the expression of NF - κB increasing, Danshen and Dachengqi Granules can decrease the NF -κB activation level.

关 键 词：慢性胰腺炎 NF—κB 细胞因子 中药 

分 类 号：R657.51[医药卫生—外科学]