冻存神经干细胞移植治疗大鼠脊髓损伤的实验研究  

The experimental study of cryopreserved neural stem cells transplanting on Wistar rat spinal cord injury

作　　者：陈君[1] 张学利[1] 江汉[1] 刘蕊[1] 郑晓莉[1] 田成瑞[1] 

机构地区：[1]天津市人民医院检验科,300121

出　　处：《中华检验医学杂志》2008年第10期1182-1185,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的研究冻存大鼠胚胎神经干细胞（NSCs）异体移植对脊髓损伤（SCI）的修复作用。方法45只Wistar大鼠于SCI后2d随机分为A、B、C 3组，每组15只，分别在SCI处注入相同体积的生理盐水、NSCs、低温冻存后复苏的NSCs。术后分别通过免疫组化和组织切片鉴定移植细胞的分化与存活、BBB运动评分和斜板实验评定大鼠功能恢复，细胞存活率，以及评价NSCs低温冻存情况。结果免疫组化及组织切片显示，B、C组在移植区均有NSCs的特异性表达nestin阳性细胞存活，而A组无表达；脊髓空洞面积A组[（3．9±0．1）mm^2]与B组[（1．2±0．3）mm^2]和C组[（1．1±0．3）mm^2]比较，组间差异有统计学意义（F=423．949，P〈0．01），而B、C组间的差异无统计学意义（P〉0．05）。BBB评分：A组[（2．0±0．5）分]与B组[（16．4±0．8）分]和C组[（16．0±1．4）分]间的差异有统计学意义（F=970．157，P〈0．01），但B、C组间差异无统计学意义（P〉0．05）；斜板实验评分：A组[（31．3±2．9）度]与B组[（46．8±2．1）度]和C组[（46．5±2．4）度]间的差异有统计学意义（F=151．099，P〈0．01），B、C组间差异无统计学意义（P〉0．05）。低温冻存后复苏液的NSCs细胞存活率[（55．9±5．2）％]与未冻存[（65．1±3．4）％]差异无统计学意义（t=3．334，P〉0．05）。结论冻存NSCs复苏后仍具有活跃的增殖和分化潜能，是SCI移植治疗有价值的细胞资源。Objective To observe the therapeutic effect of spinal cord injury （SCI） by transplantation cryopreserved neural stem cells （NSCs）. Methods 45 Wistar rats were randomly divided into three groups, with 15 rats in each group. Transplantations were carried out two days after SCI, group A： 0.9% saline water, group B： NSCs, group C： cryopreserved NSCs. After the transplantation, immunohistochemical and tissue incision were appeared to detect the survival and differentiation of the transplanted cells in nivo, and the methods of BBB and oblique plate were used to estimate the recovery of function. Results Group B and C all had active NSCs except group A. The injuried spinal cord A[ （3.9±0. 1 ） mm^2 ], B [ （ 1.2±0. 3） mm^2 ] and C [ （ 1.1±0. 3 ）mm^2 ], there was an variance in the three groups （F=423.949,P〈0.01）, BBB test： group A[（2.0±0.5） mark],B[（16.4±0.8） mark]and C [ （ 16. 0±1.4） mark], there was an variance in the three groups （ F = 970. 157, P 〈 0. 01 ）, the oblique plate test：group A[ （31.3±2.9） degree] ,B[ （46.8±2. 1） degree] and C[ （46.5±2.4） degree], there was an variance in the three groups （ F = 151. 099 ,P 〈 0. 01 ）, the tests demonstrate there were no variance in group B and C （all P 〉 0. 05 ）, there was no variance in living rate between the cryopreserved NSCs gronp [ （55.9±5.2）% ] and no cryopreserved [ （65. 1±3. 4）%, t =3. 334,P 〉0. 05]. Conclusions Cryopreserved NSCs keep the ability of reproduction and differentiation. NSCs is a kind of valuable cell resource for the therapy of SCI.

关 键 词：冻存 神经干细胞 复苏 脊髓损伤 移植 

分 类 号：R686[医药卫生—骨科学]