蛋鸡J亚群白血病病毒的分离鉴定及序列分析  

The Identification and Sequence Analysis of ALV-J Isolated from Layers

作　　者：王辉[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《病毒学报》2008年第5期369-375,共7页Chinese Journal of Virology

基　　金：国家科技支撑计划项目2006BAD06A08资助

摘　　要：通过接种鸡胚成纤维细胞(CEF)及特异性单抗的间接荧光抗体反应(IFA),从中国商品代蛋鸡群中首次分离到J亚群白血病病毒(ALV-J)。对其env基因编码的氨基酸序列及3′-末端(3′-Ter)序列与国内外来源于白羽肉鸡的毒株作了比较分析。结果显示,这两株病毒的gp85基因编码的氨基酸序列与国外5个毒株同源性仅为83.4%～87.3%,与国内来源于白羽肉鸡的8株病毒同源性也仅为86.4%～89.6%。gp37基因编码的氨基酸序列与5个国外毒株同源性为91.8%～97.0%,与8个国内毒株同源性为93.9%～95.9%。另外,国内来源于白羽肉鸡的各毒株的3′-Ter序列在"E"区均有明显缺失,但本次分离的来源于蛋鸡群的毒株在"E"区没有缺失突变。与所列出的13株国内外毒株相比,这两个毒株在3′-Ter的缺失最少,较接近于原型株HPRS-103。显然这两株病毒的来源不同于国内白羽肉鸡。Two Avian leukosis viruses of subgroup J （ALV-J） were isolated from layers by inoculating the sample into chicken embryo fibroblast （CEF） cells and by indirect fluorescent assay （IFA） with ALV-J specific monoclonal antibody JE-9. Sequence comparison indicated the gp85 identities were only in the ranges of 83.4%-87.3% compared with five international reference strains and 86.4%-89.6% compared with eight Chinese strains isolated from white meat-type chickens. The gp37 identities were in the range of 91.8-96.4% compared with the five international strains and 93.4-95.9% compared with the eight domestic strains. When compared with the above thirteen strains, two layers＂ isolates were more close to the prototype HPRS-103 and had less deletion in 3＇-Ter than those strains. All strains isolated from white meattype chickens in China had a deletion in the ＂E＂ region of 3＇-Ter except these two isolates, suggesting these two ALV-J isolates from layers have different evolution origins from other Chinese isolates from white meat-type chickens.

关 键 词：蛋鸡 J亚群白血病病毒(ALV—J) 

分 类 号：S858.31[农业科学—临床兽医学]