表达绿色荧光蛋白嵌合狂犬病病毒HEP-GFP株的拯救  

Rescue of Chimeric Rabies Virus Expressing Green Fluorescent Protein

作　　者：刘晓慧[1] 艾军[1] 郭霄峰[1] 孙京臣[1] 梁红茹[1] 

机构地区：[1]华南农业大学兽医学院微生物教研室,广州510642

出　　处：《病毒学报》2008年第5期401-403,共3页Chinese Journal of Virology

基　　金：教育部博士点基金(20060564002);广东省动物防疫检疫研究专项经费粤农[2006]264号

摘　　要：Green fluorescent protein(GFP)gene was inserted into the pseudogene(ψ)region of genome of rabies virus rHep-Flury strain,and a recombinant rabies virus carrying GFP,designated as HEP-GFP,was rescued by reverse genetics system.It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line.The research indicated that the pseudogene(ψ)region in the genome of rHEP-Flury strain,as an independent functional unit in the process of virus assembly,could independently carry and express exogenous genes.Green fluorescent protein（GFP） gene was inserted into the pseudogene （φ） region of genome of rabies virus rHep-Flury strain, and a recombinant rabies virus carrying GFP, designated as HEP-GFP, was rescued by reverse genetics system. It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line. The research indicated that the pseudogene （φ） region in the genome of rHEP-Flury strain, as an independent functional unit in the process of virus assembly, could independently carry and express exogenous genes.

关 键 词：狂犬病毒 嵌合病毒 绿色荧光蛋白 

分 类 号：S852.655[农业科学—基础兽医学]