腺病毒感染对生物燃料烟雾所致豚鼠肺部炎症反应的作用  

The effects of adenovirus 5 latent infection on pulmonary inflammation caused by biomass fumes in guinea pigs

作　　者：石喆[1] 胡国平[1] 李冰[2] 冉丕鑫[1] 

机构地区：[1]广州医学院第一附属医院广州呼吸疾病研究所呼吸疾病国家重点实验室,510120 [2]广州医学院第一附属医院广州呼吸疾病研究所实验医学研究中心,510120

出　　处：《中华结核和呼吸杂志》2008年第10期747-751,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：广东省自然科学基金研究团队资助项目（05200239）.志谢 本实验的资料分析和整理得到广州医学院组织胚胎学教研室马宁芳老师和郭晓才硕士的帮助

摘　　要：目的探讨腺病毒感染在生物燃料烟雾所致的豚鼠肺部炎症反应中的作用。方法将46只雌性白化豚鼠用随机数字表法分为感染组（26只）和假感染组（20只），感染组鼻腔接种野生型5型腺病毒（Ad5），假感染组接种PBS作为对照。22d后再用随机数字表法将每组各20只豚鼠再分为暴露于木材锯末和谷壳烟雾组与暴露于新鲜空气的对照组。21d后检测豚鼠肺组织病理和BALF中白细胞介素（IL）-8、IL-6和细胞黏附分子-1（CAM-1）的含量。所有数据均以x^-±s表示，组间比较采用单因素方差分析。结果生物燃料烟雾暴露和腺病毒感染均能使豚鼠BALF中自细胞总数明显增高[（37．1±5．5）×10^6／L和（16．8±2．3）×10^6／L]，与对照组[（11．2±2．5）×10^6／L]比较差异有统计学意义（F值分别为208．947和22．687，均P〈0．01），且有交互作用。生物燃料烟雾暴露的豚鼠BALF中IL-8、IL-6和CAM-1浓度增高[（0．38±0．06）、（0．188±0．021）和（6．5±1．6）mg／kg]，与对照组[（0．30±0．05）、（0．125±0．022）和（4．8±0．9）mg／kg]比较差异均有统计学意义（F值为13．525～69．021，均P〈0．01），腺病毒感染及豚鼠BALF中IL-8及CAM-1浓度增高[（0．37±0．05）和（8．2±2．1）mg／kg]，差异均有统计学意义（F值分别为11．964和57．162，均P〈0．01），对IL-6[（0．126±0．023）mg／kg]无明显影响（F=0．667，P〉0．05）。腺病毒感染及生物燃料烟雾暴露对豚鼠BALF中IL-8、CAM-1浓度增加有协同作用（F值分别为5．153和56．573，P〈0．05和P〈0．01）。结论木材锯末与谷壳烟雾可导致豚鼠肺部炎症反应，出现支气管炎及肺气肿样改变，腺病毒感染可加雷牛物燃料烟雾引起的豚鼠肺组织的炎症反应。Objective To explore the effects of adenovirus 5 （AdS） latent infection on oxidized injury and inflammation of the lungs caused by exposure to biomass fumes in guinea pigs. Methods Forty- six albino guinea pigs were randomly divided into 2 groups. One group （n = 26） was infected intranasally with AdS, and the other group （ n = 20） was sham-infected with sterile PBS as control. After 22 days, the living animals （n = 20 each） were randomly divided into 2 subgroups. One subgroup was exposed to biomass fumes for 3 weeks, and the other subgroup was exposed to room air. At the end of the experiment, all animals were sacrificed and their lung tissues were examined histopathologically. The levels of interleukin （IL）-8, IL-6, and cell adhesion molecule-1 （CAM-1） in bronchoalveolar lavage fluid （BALF） were measured. The results were analyzed using a two-way analysis of variance （ANOVA） with two crossed factors. The level of significance was P 〈 0.05. Results The exposure to biomass fumes and the latent infection of AdS were associated with a significant increase in the total number of airway inflammatory cells in the BALF[ （37. 1 ±5.5 ） ×10^6/L and （ 16. 8 ±2. 3） ×10^6/L] ,F = 208. 947, 22. 687, all P 〈 0. 01 ）. The exposure to biomass fumes was associated with a significant increase in the concentration of IL-8, IL-6, and CAM-1 in the BALF[ （0. 38 ±0.06）, （0. 188 ±0. 021） and （6. 5 ±1.6） mg/kg] ,F = 13. 525 -69. 021, all P 〈0. 01 ）. The latent infection of Ad5 was associated with a significant increase in the concentration of IL-8, and CAM-1 in the BALF[ （0. 37 ±0. 05） and （8.2 ±2. 1 ） mg/kg] （F = 11.964, 57. 162, all P 〈 0. 01 ）. Ad5 infeetion had a synergistic effect on the IL-8 and CAM-1 production caused by biomass fume exposure. Conclusions Biomass fumes caused severe histopathological changes and inflammation in lungs of guinea pigs, and Ad5 latent infection aggravated these changes. Increased production of inflammatory eytokines ma

关 键 词：烟雾吸入损伤 腺病毒科感染 肺炎 豚鼠 

分 类 号：R686[医药卫生—骨科学]