间充质干细胞与人脐血CD34^＋细胞共移植对NOD／SCID小鼠造血重建的影响  

Study on hematopoiesis reconstitution by co-transplant of human bone marrow mesenchymal stem cells and mnbmcai cord blood CD34^＋ cells at different ratios in NOD/SCID mice

作　　者：马俐君[1] 胡晓霞[1] 周虹[1] 高磊[1] 解琳娜[1] 邱慧颖[1] 王健民[1] 

机构地区：[1]第二军医大学附属长海医院血液科,上海200433

出　　处：《中华血液学杂志》2008年第10期684-688,共5页Chinese Journal of Hematology

基　　金：国家“863”高技术发展项目（2002AA205051）;上海市科委重大基础研究基金（03DJ14020）

摘　　要：目的观察间充质干细胞（MSC）与不同比例脐血CD34^＋细胞共移植对NOD／SCID小鼠造血重建的影响，明确MSC与脐血CD34^＋细胞共移植的最适数量。方法给^60Coγ射线照射的雌性NOD／SCID小鼠共移植人MSC和不同比例的脐血CD34^＋细胞，观察共移植后42d内小鼠外周血白细胞和血小板变化，并于移植后42d处死小鼠，用流式细胞术检测外周血、骨髓和脾脏人源细胞含量。结果与单纯脐血CD34^＋细胞移植相比较：①脐血CD34^＋细胞与1、5和10倍数量的MSC共移植时，可明显减轻外周血白细胞和血小板的下降幅度（P〈0．01），提前I周使白细胞和血小板恢复至正常水平（P〈O．05），三组间差异无统计学意义（P〉0．05）；②MSC与不同比例的脐血CD34^＋细胞共移植均可明显提高外周血、骨髓和脾脏造血细胞植入率，比例为10：1时，外周血、骨髓和脾脏中的人源细胞（huCD45^＋细胞）含量分别增加了（2．8±0．6）倍、（3．5±0．9）倍和（5．2±0．6）倍，增加倍数差异均有统计学意义（P〈0．01），达到了最佳的植入效果。结论脐血CD34^＋细胞与10倍数量的MSC共移植可达到最佳的促进造血重建作用。Objective To study the effect of co-transplant of human bone marrow mesenchymal stem cells （BMMSCs） and umbilical cord blood （UCB） CD34^＋ cells on hematopoiesis reconstruction in NOD/ SCID mice and to investigate the optimal proportion between the two kind of cells. Methods Female NOD/ SCID mice were sublethaily irradiated by ^60Coγ-ray and transplanted with BMMSCs and different ratios of UCB CD34^＋ cells. From day ＋ 3 till day ＋ 42 after transplantation, 20 μl peripheral blood （PB） was collected from the retro-orbital plexus of mice weekly, and the variations of WBC and PLT were counted. Mice were sacrificed 42 days after transplantation, and human CD45 positive （ huCD45 ^＋ ） cells in PB, BM, and spleen were detected by flow cytometry. Results Compared with transplant of UCB CD34^＋ ceils alone, cotransplatation of BMMSCs and UCB CD34^＋ cells at ratios of 1： 1,5： 1 and 10： 1 ,①significantly mitigated the decrease range （P〈 0. 01）and led to the recovery of WBC and platelet in PB one week earlier（P 〈 0.05 ）, and the difference among the three groups was not statistically significant （P 〉 0.05 ） ;②significaantly enhanced hematopoietic stem cells （PB, BM and spleen cells） engraftment in recipient mice, and the effect was most pronounced at the ratio of 10： 1. huCD45 ^＋ cells in PB, BM and spleen were increased by （2.75 ± 0.63）, （3.51±0.86） and （5.18 ±0.57）fold, respectively （P〈0. 01）. Conclusion The optimal hematopoiesis reconstruction is achieved by co-transplant of UCB CD34^＋ cells and BMMSCs at a ratio of 1 ： 10.

关 键 词：间充质干细胞 胎血 CD34’细胞 NOD/SCID小鼠 造血重建 

分 类 号：R686[医药卫生—骨科学]