航天诱变番茄无限生长突变体RAPD及SCAR分子标记研究  

RAPD and SCAR Markers Analysis on the Indeterminate Growth Mutant of Tomato Derived by Space Mutagenesis

作　　者：王艳芳[1] 王世恒[2] 赵彦宏[3] 郑积荣[2] 祝水金[1] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310029 [2]杭州市蔬菜科学研究所,浙江杭州310024 [3]鲁东大学生命科学学院,山东烟台264025

出　　处：《航天医学与医学工程》2008年第5期407-410,共4页Space Medicine & Medical Engineering

基　　金：浙江省科技重点项目(2004C22014);杭州市科技重大项目资助(2003122B14)

摘　　要：目的通过对航天诱变所获得的番茄无限生长习性突变体进行分子水平的鉴定,为番茄生长习性分子标记选育提供依据。方法用50个10-mer随机护增多态性DNA(randomly amplified polymorphic DNA,RAPD)RAPD引物检测M1和10-3-2基因组序列多态性,对多态性片断回收克隆后转化成序列特征性扩增区域(sequence characterized amplified region,SCAR)标记。结果50个RAPD引物中有44个引物扩增出产物,其中2个引物(S165和S168)扩增出稳定的重复性好的多态性条带,均表现为M1缺失条带。其特异扩增产物分子量分别为300bp和1500bp,暂命名为TRS165300和TRS1681500,其中TRS1681500标记已经转换成了稳定的SCAR标记,并可作为该突变体的特异遗传标记。结论航天诱变可以从DNA水平对搭载材料进行诱变,通过航天诱变获得的番茄无限生长习性突变体,为研究番茄生长习性调控提供了宝贵的材料。Objective To evaluate the indeterminate growth mutant of tomato derived by space mutagenesis to provide the basis for selecting and cultivating the molecular markers of tomato growth habit. Methods Fifty 10-mer randomly amplified polymorphic DNA（RAPD） primers were used to examine the polymorphism of M1 and 10-3-2. Their polymorphic fragments were cloned, and then were transferred to SCAR markers. Results Of all the 50 10-mer RAPD primers, 44 primers amplified polymerase chain reaction（PCR） products and 2 primers （S165 and S168） amplified stable reproducible polymorphic products. The molecular weight of the specific amplified products were 300 bp and 1 500 bp respectively, therefore they were named as TRS165300 and TRS1681500 temporarily. And TRS1681500 was transferred into stable sequence characterized amplified region（SCAR） marker and this marker could be a specific genetic marker of this indeterminate growth habit mutant. Conclusion Space mutation can produce mutants at DNA level from the loaded materials. The indeterminate growth mutant of tomato is derived by space mutagenesis which can provide a valuable material for studying the regulation and control of tomato growth habit.

关 键 词：航天诱变 番茄 无限生长突变 随机扩增多态性DNA 序列特征性扩增区域 

分 类 号：O693[理学—化学]