阿司匹林对人γδT细胞杀伤消化系统肿瘤细胞的影响  

The effect of aspirin on human's γδT cells killing digestive system tumor cell lines

作　　者：刘军权[1] 陈复兴[1] 朱斌[2] 张颂[1] 张娟[1] 陶征中[1] 李佚[1] 

机构地区：[1]解放军第97医院实验科,江苏徐州221004 [2]解放军第97医院药剂科,江苏徐州221004

出　　处：《细胞与分子免疫学杂志》2008年第10期966-968,971,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：南京军区医学科学技术研究"十一五"计划课题(06MA45)

摘　　要：目的:探讨阿司匹林对人γδT细胞杀伤消化道肿瘤细胞株的影响。方法:用异戊烯焦磷酸法体外扩增人外周血γδT细胞。用不同浓度的阿司匹林诱导γδT细胞和消化道肿瘤SGC-7901、SW-1990、SW-480、SW-1116、LOVO细胞株,用乳酸脱氢酶法测定γδT细胞的杀伤活性,用流式细胞术(FCM)检测阿司匹林诱导前后的γδT细胞和SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞凋亡百分率。结果:γδT细胞培养10d时从扩增前4.21%增加到70.35%。阿司匹林0.4～0.8mmol/L诱导24h后的γδT细胞对5种肿瘤细胞的杀伤活性最高,浓度超过3.2mmol/L时杀伤活性呈下降趋势;SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞经不同浓度的阿司匹林诱导24h后除药物浓度在0.4mmol/L时γδT细胞对SW-480、SW-1116和LOVO细胞株的杀伤活性略有增强外,其他组与对照组比较无明显变化。3.2mmol/L阿司匹林对γδT细胞诱导24h的凋亡率(52.71%)明显高于SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株(分别为7.88%、8.89%、6.21%、4.47%和%3.67)。结论:阿司匹林在临床常规使用的药物浓度时可增强γδT细胞杀伤肿瘤细胞作用,超过这一浓度可明显抑制γδT细胞的增殖能力和杀伤活性及增加γδT细胞的凋亡率,而对SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株作用不明显。AIM：To explore the effect of aspirin on human＇s γδT cells killing digestive system tumor cell lines.METHODS：Use the isopentenyl pyrophosphate method to amplify human peripheral blood γδT cells in vitro.Various concentrations of aspirin were used to induce γδT cells and digestive system tumor cells SGC-7901,SW-1990,SW-480,SW-1116,LOVO cells lines,LDH assays was used to measure the cytotoxic activity of γδT cells,flow cytometry analysis the apoptosis percentage of before and after induced γδT cells and SGC-7901,SW-1990,SW-480,SW-1116,LOVO cell lines.RESULTS：γδT cells were cultivated for ten days which proliferation ratio increase from 4.21% to 70.35%.When γδT cells induced via aspirin concentrations rang from 0.4 mmol/L to 0.8 mmol/L which cytotoxic activity on the five kinds of tumor cell lines was the highest,if aspirin＇s concentration surpassing 3.2 mmol/L,cytotoxic activity of γδT cells present decrease tendency.SGC-7901,SW-1990,SW-480,SW-1116,LOVO cells lines were induced by different concentrations of aspirin for 24 hours,just SW-480,SW-1116 and LOVO were enhanced as far as the cytotoxic activity of γδT cells on these cell lines was concerned,other groups and control group have no notable changed.The apoptosis percentage of γδT cells（52.71%）were induced by aspirin which concentration was 3.2 mmol/L,which was strikingly higher than SGC-7901,SW-1990,SW-480,SW-1116,LOVO cells lines,（respectively 7.88%,8.89%,6.21%,4.47% and 3.67%）.CONCLUSION：When aspirin＇s concentration for clinical routine used can enhance the effect of γδT cells killing the tumor cells,if surpassing this concentration can obvious inhibited the proliferation capacity and cytotoxic activity of γδT cells and augment apoptosis ratio,however,it is not obvious for digestive tract tumor lines.

关 键 词：ΓΔT细胞 阿司匹林 消化系统肿瘤细胞株 

分 类 号：R392.12[医药卫生—免疫学]