猪伪狂犬病病毒gC基因的克隆及其部分生物学特性的分析  

Cloning of gC gene of porcine pseudorabies viruses and analyses of its partial biological characteristics

作　　者：王键义[1] 郭万柱[1] 徐志文[1] 王印[1] 王小玉[1] 

机构地区：[1]四川农业大学动物医学院动物生物技术中心,四川雅安625014

出　　处：《中国兽医科学》2008年第10期830-836,共7页Chinese Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"项目(IRTO555);国家"十一五"科技支撑计划项目(2006BAD06A18)

摘　　要：采用PCR方法扩增出12株伪狂犬病病毒（PRV）的gC基因全序列，并测序，使用生物学软件对这12株PRVgC基因序列和GenBank上登录的6条gC基因序列进行了生物信息学分析和预测。结果显示，PRVgC基因开放阅读框的核苷酸长度为1437～1464bp，编码478～487个氨基酸。在遗传进化关系上，四川省流行的PRV毒株与日本、欧洲、美洲分离株的亲缘关系较近，而与我国其他地区的分离株亲缘关系较远。这些毒株的蛋白疏水性、抗原表位和高级结构等具有相似性，但也存在一些变化和差异。表明，PRVgC基因具有较高的保守性，但可能存在抗原漂移现象。The full-length of gC gene 12 pseudorabies virus（PRV） strains were amplified by PCR,and sequenced. Then, they were analyzed and predicted with 6 gC gene sequences available in GenBank by using bioinformatic softwares. Open reading frame of the amplified gC gene was 1 437 to 1 464 bp,encoding 478 to 487 amino acids correspondingly. The PRV strains prevalent in Sichuan Province were genetically close to the PRV strains isolated in Japan, Europe and America, but distant to isolates in other regions of China. There were resemblance in proteo-hydrophobicity, epitopes and three dimentional structures with the ex ception of subtle difference among the 12 strains of PRV. The results showed that the gC gene was highly conserved, but might take antigenic drifts.

关 键 词：伪狂犬病病毒 GC基因 PCR 生物学特性 

分 类 号：S852.659.1[农业科学—基础兽医学]