TSOL18抗原重组减毒鼠伤寒沙门菌活载体疫苗对小鼠的免疫保护作用  

Immune protection of recombinant attenuated Salmonella typhimurium live vector vaccine expressing TSOL18 antigen for mice

作　　者：丁军涛[1] 王颖[1] 陈晓宇[1] 骆学农[1] 郑亚东[1] 景志忠[1] 才学鹏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2008年第10期851-855,共5页Chinese Veterinary Science

基　　金：国家高技术研究发展计划(863)项目(2006AA10A207);国家"十一五"科技支撑计划项目(2007BAD40B03)

摘　　要：用构建好的活载体疫苗X4550(pYA3341-TSOL18)免疫小鼠,检测小鼠血清中IgG、IgM和肠液中IgA抗体的产生及各免疫组织重组疫苗株的分布情况,分析小鼠脾细胞亚型的分化,探讨了重组疫苗的免疫保护效果。结果显示,在免疫后第7 d,小鼠血清中有抗TSOL18 IgM产生,在二免后第28 d,IgG的D492 nm值达到0.645,在二免后第42 d,肠液中有抗TSOL18分泌性IgA抗体产生,表明重组疫苗能诱导小鼠产生较强的抗体水平和激发多种方式的免疫应答;小鼠免疫后第21 d在脾中仍能检测到大量的重组疫苗株,表明TSOL18重组蛋白的表达没有影响重组疫苗X4550(pYA3341-TSOL18)的定居能力;免疫小鼠的CD4+、CD8+T淋巴细胞数目明显增多,CD4+/CD8+T细胞比值也显著增高(P<0.01),提示重组疫苗可能通过提高CD4+/CD8+T细胞比值来发挥免疫作用。Mice were immunized with the constructed recombinant attenuated Salmonella typhimurium live vector vaccine X4550（pYA3341-TSOI,18） to evaluate the efficacy of the vaccine. The mouse sera IgG and lgM antibodies and the mouse liquor entericus secreting IgA antibody were examined by ELISA. The recombinant vaccine bacteria persisting in mice immune tissues were examined. The splenocyte subsets were assayed. The IgM antibody could be detected on day 7 post-immunization（PI） and the D490nm of IgG antibody could reach to 0. 645 on day 28 post-2nd-immunization（P2I） and the secreted IgA could be detected in mouse liquor entericus on day 42 P2I. The vaccine could induce production of the antibodies including IgM,IgG and IgA. The vaccine could be detected in mice spleen on day 21 PI. Both the amounts of CD4＋ and CD8＋ T lymphocytes and the proportion of CD4＋ to CD8＋ T lymphocytes were increased significantly in the mice immunized with the vaccine X4550 as compared with those in the mice of control groups,indicating that the expression of recombinant TSOL18 protein did not affect ecesis of the recombinant vaccine . The results revealed that the vaccine may build a immunifaction by elevating the proportion of CD4＋/CD8＋ T cells and could induce Thl and Th2 immunity responses at the same time.

关 键 词：活载体疫苗X4550 小鼠 免疫保护 酶联免疫吸附试验 T淋巴细胞 

分 类 号：S852.612[农业科学—基础兽医学] S852.4[农业科学—兽医学]