猪霍乱沙门菌SC500运载TGEVS与猪IL-15融合基因疫苗的稳定性及免疫安全性  

Stability and immunizing-safety of TGEV-S-IL-15 gene vaccine using Salmonella choleraesuis SC500 as transporter

作　　者：韩国全[1] 郭万柱[1] 孙志勇[1] 王印[1] 王小玉[1] 林华[1] 

机构地区：[1]四川农业大学动物医学院动物生物技术中心,四川雅安625014

出　　处：《中国兽医科学》2008年第10期871-877,共7页Chinese Veterinary Science

基　　金：教育部长江学者和创新团队发展计划项目(IRT0555)

摘　　要：为研究猪霍乱沙门菌SC500作为TGEV S与IL-15融合基因疫苗pCC-S-IL-15、pCI-S-IL-15运载体的稳定性与免疫安全性,将外源表达质粒pCC-S-IL-15、pCI-S-IL-15及空载体质粒pCC-neo、pCI-neo转化SC500,采用体外增殖试验研究不同质粒在SC500中的稳定性,携带质粒的SC500对ST细胞的黏附率、侵袭力和增殖能力的影响;采用BALB/c小鼠口服免疫试验研究表达质粒在体内的稳定性及SC500对小鼠的安全性。结果显示:外源目的基因影响表达载体在运载体内的稳定性,就载体类型而言,对pCI-neo的影响大于对pCC-neo的影响;携带目的基因的外源质粒影响SC500对ST细胞的黏附率和侵袭率,pCC-neo影响大于pCI-neo;外源质粒影响SC500在小鼠体内的增殖能力,pCC-neo影响大于pCI-neo;SC500在体内的增殖中,其所运载的质粒具有一定的稳定性。结果表明,利用SC500运载基因疫苗pCC-S-IL-15、pCI-S-IL-15免疫小鼠具有相对的稳定性和免疫安全性。To investigate the stability and safety of fusion-expression gene vaccines pCC-S-IL-15 and pCI-S-IL-15 with TGEV-S and porcine IL-15 genes using Salmonella choleraesuis SC500 strain as trans porter,the exogenous expression plasmids pCC-S-IL-15, pCI-S-IL-15, empty plasmid pCC neo and pCI neo were transformed into SC500. The stability of plasmids in the transporter was studied by the in vitro proli feration of SC500. The effects of SC500 carrying the plasmids on adhesiveness,invasiveness and proliferation of SC500 to ST cells was studied. The immunity experiments on BALB/c mice were carried out to investigate the stability in vivo of these plasmids and the security of SC500 in mice. The expression vector pCI-neo in the transporter was impacted stronger by exogenous genes than by pCC neo. The ratio of adhe siveness and invasiveness of SC500 to ST cells was affected by exogenous plasmids and it was stronger for pCC-neo than pCI-neo. Exogenous plasmids influenced the reproductive activity of SC500 in vivo. As to the type of the vectors,the action of pCC-neo was more powerful than pCI-neo. The plasmids transported into SC500 possessed certain stability when SC500 was proliferated in vivo. The results showed that SC500 was safer and steadier than the transporter of pCC S-IL-15 and pCI-S-IL-15.

关 键 词：猪霍乱沙门菌 TGEV—S基因 猪白细胞介素15基因 稳定性 免疫安全性 

分 类 号：S852.612[农业科学—基础兽医学]