硝基苯对小鼠肝微粒体混合功能氧化酶活性的影响  

Toxic effect of nitrobenzene on the liver in mice

作　　者：邢厚娟[1] 刘伯臣 徐世文[1] 丁岚峰 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030 [2]黑龙江民族职业技术学院,黑龙江哈尔滨150081

出　　处：《中国兽医科学》2008年第10期899-903,共5页Chinese Veterinary Science

基　　金：黑龙江省教育厅资助项目(YJSCX2007-0043HLJ)

摘　　要：采用灌胃的方法对试验小鼠进行硝基苯(NB)染毒,染毒剂量分别为26 mg/kg、52 mg/kg、105mg/kg,每天染毒1次,共30 d,观察小鼠肝显微及超微结构的变化,并检测了肝微粒体混合功能氧化酶活性。结果显示,主要表现为光镜下肝细胞水变性,电镜下线粒体肿胀,脊断裂;内质网脱核糖颗粒;细胞核发生形变;次级溶酶体、脂滴数量增多,糖原颗粒减少等一系列的病理学变化。随着染毒剂量的加大,肝微粒体混合功能氧化酶中的细胞色素P450的含量呈波动性变化;细胞色素b5和EROD酶的活性逐渐升高;NAD-PH细胞色素C还原酶的活性逐渐降低。表明NB对小鼠肝毒性作用随染毒剂量的增加而加强;EROD可以作为检测环境中NB污染水平的生物标志物。Nitrobenzene（NB） of 26 mg/kg,52 mg/kg and 105 mg/kg was administered intragastricly and daily into 3 groups of mice for 30 d,respectively and the effect of NB on rnicrostructure and ultrastructure of the mice liver were discussed. The water-apomorphosis of liver cells was observed under the light microscope. The histopathological changes of ultrastructure included mitochondrion swelling,lophos-breakage, ribosome dislocating endoplasmic reticulum, cellular nucleus deformation, the second lysosome and lipid granules increasing and glycogen granules decreasing. Cytochrome P450 representing microsomal mixed- function oxidase in liver was observed to be undulate quantitated changes. Cytochrome bS,arnid-pyrylium- N-demethylase,EROD and 7-ethoxy-cumarin O deoxethyl enzyme activities were increased with an increase in exposure dosages, while the activity of NADPH-cytochrome C reductases was decreased. The results showed that NB was remarkably toxic in liver and the toxicity would increase with the increased exposure doses. EROD could be used as an index to quantitate NB in environment detection.

关 键 词：硝基苯 肝 显微结构 超微结构 细胞色素P450 

分 类 号：S859.82[农业科学—临床兽医学]