抗生素对组培条件下植物技原体作用的初探  

Seek for Effects of Antibiotics on Phytoplasma in Tissue Culture

作　　者：李加友[1] 

机构地区：[1]南京农业专科学校园艺系,南京210038

出　　处：《南京农专学报》1997年第4期25-31,共7页Journal of Nanjing Agricultural Technology College

摘　　要：采用3种国产医用抗生素（盐酸－四环素、交沙霉素、枝原清）以不同浓度处理组培条件下的泡桐丛核病组培苗和甘薯丛枝病组培苗。结果表明：15μg／ml盐酸－四环素对泡桐丛枝病组培苗连续作用60d，病株外观基本恢复正常，再连续作用60d，症状无复发，经电镜观察未发现植物枝原体的存在。2．5μg／ml盐酸－四环素对甘薯丛枝病效果最好，对处理60d的甘薯组培前做电镜观察，未发现植物枝原体的存在。交沙霉素和技原清均对泡桐丛枝病有一定效果，但交沙霉素对泡桐丛枝病组培苗，枝原清对泡桐丛技病组培苗和甘薯丛枝病组培苗均有毒害作用。Three kinds of medicina1 antibiotics, tetracycline hydrochloride, tylosin and josamycinum, were used to treat Paulownia witches' broom disease and Ipomoea batata witches' broom disease with different concentrations in tissue culture. the results of this study show: Diseased Paulownia seedlings were treated 60days with tetracycline hydrolchloride of 15 μg/ml, the appearance of diseaed seedlings returned to normalcy. After a second 60-day treatment, no symptoms returned and no Phytoplasma was observed through electron microscope. No Phytoplasma was observed in phloem tissues of diseased ipomoea batata seedlings which had been treated 60 days with tetracycline hydrochloride of 2. 5 μg/ml, too.Josamycinum and tylosin had a certain treatment effect on the two kinds of desease caused by Phytopasma, but they did some harm to seedlings.

关 键 词：盐酸四环素 交沙霉素 丛枝病 植物枝原体 

分 类 号：S432.9[农业科学—植物病理学] S763.724.3[农业科学—农业昆虫与害虫防治]