一种高效稳定的甘蓝染色体制片新方法  

An Efficient and Stable Method For Chromosome Flaking in Brassica oleracea

作　　者：卢军[1] 李乐玉[1] 朱利泉[1] 王小佳[1] 

机构地区：[1]西南大学农学与生物科技学院,重庆400716

出　　处：《北方园艺》2008年第9期148-150,共3页Northern Horticulture

基　　金：国家自然科学基金资助项目(30671429)

摘　　要：介绍一种高效稳定的甘蓝根尖染色体制片新方法,该方法结合传统的压片法和去壁低渗法优点,制片取得了良好的结果:染色体中期分裂相多,染色体分散良好,形态特征清晰,背景干净。制片可应用于染色体核型分析、分带、荧光原位杂交,以及显微切割等细胞遗传学操作。An efficient and stable method for chromosome flaking of Brassica oleracea toot-tip has been developed.This method integrated the disadvantage of squashing method and wall digestion hypotonic method,the primary results showed that the modified process could easily get more metaphase cells,satisfying background,well decentraztion and clear morphological chromosome.these chromosomal specimens were fitted to be used in such cytogenetics manipulations as karyotyping,banding,fluorescence in situ hybridization and microdissection.

关 键 词：甘蓝 染色体 制片 

分 类 号：S635[农业科学—蔬菜学] Q94-331[农业科学—园艺学]