猪精液冷冻技术的研究  

Study on Frozen Technique of Pig Semen

作　　者：麻常胜[1] 张兆旺[1] 王金勇[2] 蔡元 潘红梅[2] 

机构地区：[1]甘肃农业大学动物科技学院,兰州730070 [2]重庆市畜牧科学院,荣昌402460 [3]兰州畜牧兽医研究所,兰州730050

出　　处：《青海畜牧兽医杂志》2008年第5期9-11,共3页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：重庆市"十一五"动植物良种创新工程项目资助(CSTC2006AA1008)

摘　　要：从稀释液、解冻液、解冻速率方面研究了猪精液冷冻保存的效果。结果表明:在八种稀释液中,用Ⅰ号稀释液制作的冷冻精液解冻后精子活率(33±1.2)%优于其它组(P<0.05);Ⅴ号解冻液解冻后精子活率(34±2.4)%极显著高于Ⅰ、Ⅲ、Ⅳ号解冻液(P<0.01),Ⅴ号解冻液解冻后精子存活时间506.6±40.4h,极显著高于Ⅰ、Ⅱ、Ⅲ号解冻液(P<0.01);以27.5℃/s的速率解冻,解冻后精子各项指标均为最高值。The effect of frozen storage of pig semen was studied in the semen diluent, thawing solution and thawing rate. The results showed that in eight semen diluents,the sperm motility rate after thawing of frozen semen manufactured with semen diluent Ⅰ was （ 33 ± 1.2 ） % which was significantly higher than that of other semen-diluents （ P 〈 0.05 ） ; the sperm motility rate after thawing with the thawing solulion Ⅴ was （34 ± 2.4 ） % which was very sighificantly higher than that of the thawing solution Ⅰ , Ⅲ and Ⅳ（ P 〈 0.01 ） and the sperm survival time of them was 506.6 ± 40.4h which was very significantly higher that of the thawing solution Ⅰ, Ⅱ and Ⅲ （ P 〈 0. 01）. The each sperm parameter after thawing was the best when the thawing rate was 27.5%.

关 键 词：猪 冷冻精液 稀释液 解冻速率 

分 类 号：S821.2[农业科学—畜牧学]