染砷大鼠生精细胞凋亡及其Bcl-2表达的变化  

The Effects of As_2O_3 on Apoptosis and Bcl-2 Expression of Rat Spermatogenic Cell

作　　者：陈伟[1] 陆祥[1] 舒小林[1] 颜彦[2] 

机构地区：[1]遵义医学院组织学与胚胎学教研室,563003 [2]遵义医学院

出　　处：《贵州医药》2008年第10期870-872,F0003,共4页Guizhou Medical Journal

基　　金：贵州省科技厅国际合作重点项目资助[黔科合J字(2006)2078]

摘　　要：目的观察不同剂量三氧化二砷(As2O3)对大鼠生精细胞凋亡及其Bcl-2表达的变化。方法40只健康雄性SD大鼠随机分成四组,分别为0.375、0.75、1.5mg/kg3个染毒组和对照组,灌胃法连续给药16周后,采用末端转移酶介导的dUTP缺口末端标记法(TUNEL)法检测大鼠生精细胞凋亡,免疫组化法检测大鼠生精细胞Bcl-2的表达。结果(1)与对照组相比,生精细胞凋亡指数(AI)显著升高(P<0.01),生精细胞Bcl-2阳性产物表达明显降低(P<0.01)。(2)每日精子生成量与生精细胞凋亡呈负相关(r=-0.563,P<0.01)。(3)生精细胞凋亡与Bcl-2阳性表达呈负相关(r=-0.737,P<0.01)。结论一定剂量的As2O3可通过抑制生精细胞Bcl-2的表达,促进生精细胞凋亡而导致精子生成量的减少,产生雄性生殖毒性。Objective To investigate the occurrence of germ cell apoptosis and the expression of Bcl-2 after aniso-doses arsenic （As2 O3） administration in the testes of the adult male rats. Methods 40 healthy male Sprague-Dawle rate were divided randomly in. to four groups and they were administered respectively with 0 （control group）,0. 375,0. 75,1.5 mg/kg body weight of As2O3 by introgastric administration consecutively for 16 weeks. The apoptosis of germ cell were assessed by in situterminal deoxynucleotityl transferase mediated dTUP nick end labeling （TUNEL） technique. The expression of Bcl-2 in spermatogenic cells of different grades were located and quantitated by method of immunohis-tochemistry. Results （1）The apoptosis index of spermatogenic cells （AI） significantly increased compared with that in the control group （P〈0.01）;Bcl-2 expression of middle dose group and high dose group decreased significantly compared with that in the control group （P〈0. 01）. （2）The dependability analysis between daily sperm reproduction （DSP） and AI showed a negative correlation （r=- 0. 563, P〈0. 01）. （3） The negative correlation was significant between AI and Bcl-2 expression of spermatogenie cells （r=- 0. 737, P〈0. 01）. Conclusion One mechanisms of male reproduction toxicity of As2O3 might be that Bcl-2 expression is inhibited, which induces decreases of quantity of sperm cells.

关 键 词：三氧化二砷 凋亡Bcl-2 生精细胞 大鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学] R339.2[医药卫生—基础医学]