毛细管电泳高频电导法测定水线草中齐墩果酸和熊果酸的含量  

Determination of Oleanolic Acid and Ursolic Acid in Hedyotis corymbosa by Capillary Zone Electrophoresis with High Frequency Conductivity Detection

作　　者：朱培仪[1] 宋粉云[1] 刘春霞[2] 

机构地区：[1]广东药学院,广州市510224 [2]中山大学附属第二医院药学部,广州市510120

出　　处：《中国药房》2008年第30期2356-2358,共3页China Pharmacy

基　　金：广东省自然科学基金资助项目(5002841)

摘　　要：目的:建立水线草中齐墩果酸和熊果酸的含量测定方法。方法:采用毛细管区带电泳、高频电导检测:未涂层弹性融硅石英毛细管柱(55cm×75μmID),有效长度50cm;以1.2mmol·L-1三乙胺-HCl(pH10.0)、0.24mmol·L-1β-环糊精为运行缓冲液,分离电压12.5kV,重力进样10s(高度20cm)。布洛芬为内标。结果:齐墩果酸、熊果酸的检测浓度分别在3.9～39.0μg·mL-1(r=0.999 1)、20.0～140.0μg·mL-1(r=0.999 0)范围内与各自峰面积积分值呈良好的线性关系;平均回收率分别为96.5%、96.0%,RSD分别为1.98%、1.09%(n=6)。结论:本方法简便、准确、快速、重现性好,可用于水线草的质量控制。OBJECTIVE： To establish a method for the determination of Oleanolic acid and Ursolic acid isomers in Hedyotis corymbosa.METHODS, Capillary zone electrophoresis with high frequency conductivity detection was adopted. The separation of sample was performed on an uncoated fused silica capillary（55 cm ×75μm ID） at an effective length of 50 cm. 1.2 mmol· L^-1 Triethylamine（TEA） -HCl（pH10.0） buffer containing 0.24 mmol·L^-1β- cyclodextrin was selected for the running buffer solution.The voltage applied was 12.5 kV and the sample was injected by gravity for 10 s at a height of 20 cm. Brufen was used as internal standard.RESULTS： The linear ranges for Oleanolic acid and Ursolic acid were 3.9-39.0μg · mL^-1 （r = 0.999 1） and 20.0-140.0μg · mL^- 1（ r = 0.9990）, respectively, and their average recovery rates were 96.5% and 96.0% respectively and RSD were 1.98% and 1.09%, respectively（ n =6）.CONCLUSION： h was proved that the method was simple, accurate, rapid and reproducible, and applicable for the quality control of Hedyotis corymbosa.

关 键 词：毛细管电泳 高频电导检测 水线草 齐墩果酸 熊果酸 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]