干蟾皮质量标准研究  

Quality Standard of Bufo gargarizans

作　　者：庄美芳[1] 梁桃圣 

机构地区：[1]江苏南通市中医院,南通市226001 [2]江苏南通精华制药股份有限公司,南通市226005

出　　处：《中国药房》2008年第30期2363-2365,共3页China Pharmacy

摘　　要：目的:建立干蟾皮的质量标准。方法:采用薄层色谱(TLC)法定性鉴别干蟾皮;采用高效液相色谱法测定华蟾酥毒基和脂蟾毒配基的含量。结果:TLC中斑点清晰,分离度好;华蟾酥毒基和脂蟾毒配基的进样量分别在0.100 4～1.255 0μg(r=0.999 9)、0.103 6～1.295 0μg(r=0.999 9)范围内与各自峰面积积分值呈良好线性关系;二者平均回收率分别为98.35%、98.26%,RSD分别为1.48%(n=9)、1.76%(n=9)。结论:所建标准可用于干蟾皮的质量控制。OBJECTIVE： To establish the quality standard of Bufo gargarizans .METHODS： The quality of Bufo gargarizans was identified by TLC and the contents of Cinobufagin and Bufogenin were determined by HPLC.RESULTS： The TLC spots were clear and well - isolated.The linear ranges of Cinobufagin and Bufogenin were 0.100 4-1.255 0 μg（ r =0. 999 9） and 0.103 6-1.295 0 μg （r = 0.999 9）, respectively, with average recovery rates at 98.35% and 98.26%, respectively and RSD at 1.48% （n =9） and 1.76% （n = 9）, respectively. CONCLUSION： The established standard is suitable for the quality control of Bufo gargarizans.

关 键 词：干蟾皮 薄层色谱法 高效液相色谱法 质量标准 

分 类 号：R284.1[医药卫生—中药学] R927.1[医药卫生—中医学]