Spectrophotometric Determination of Arginine in Grape Juice Using 8-Hydroquinoline  

作　　者：WANG Hua LIANG Xin-hong ZHAO Rui-xiang FENG Li-dan LI Hua 

机构地区：[1]College of Enology, Northwest A ＆ F University, Yangling 712100, P.R.China [2]School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, P.R.China [3]Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, P.R.China

出　　处：《Agricultural Sciences in China》2008年第10期1210-1215,共6页中国农业科学（英文版）

基　　金：the National Science and Technology Achievements Spreading Programme from the Ministry of Science and Technology of China(2004EC000317)

摘　　要：Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, known as carcinogen. The aim of this study was to develop a simple, fast, and accurate method for determining arginine in grape juice with Sakaguchi reaction by separating arginine with strong cation-exchange resins. Parameters were optimized including the concentrations of 8-hydroquinoline and sodium hydrobromite. The color stability lasted for 4 min, which is sufficient to finish the measurement. The method is simple, reproducible and accurate, and can be applied for quick measurement of arginine in grape juice to take necessary measures for controlling the level of ethyl carbamate.Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, known as carcinogen. The aim of this study was to develop a simple, fast, and accurate method for determining arginine in grape juice with Sakaguchi reaction by separating arginine with strong cation-exchange resins. Parameters were optimized including the concentrations of 8-hydroquinoline and sodium hydrobromite. The color stability lasted for 4 min, which is sufficient to finish the measurement. The method is simple, reproducible and accurate, and can be applied for quick measurement of arginine in grape juice to take necessary measures for controlling the level of ethyl carbamate.

关 键 词：grape juice L-ARGININE ethyl carbamate Sakaguchi reaction 

分 类 号：TS275.5[轻工技术与工程—农产品加工及贮藏工程]