自发性高血压大鼠血管壁脂联素的表达及其意义  

Expression and significance of adiponectin in vascular walls of spontaneously hypertensive rats

作　　者：朱俐俐[1] 刘庚勋[1] 郭立新[1] 

机构地区：[1]解放军第163医院,湖南长沙市410003

出　　处：《心血管康复医学杂志》2008年第5期447-450,共4页Chinese Journal of Cardiovascular Rehabilitation Medicine

摘　　要：目的:探讨自发性高血压大鼠(SHR)血管壁脂联素的的表达和福辛普利、氯沙坦对其表达的影响。方法:22周龄20只SHR大鼠随机被分成四组:福辛普利干预组、氯沙坦干预组、福辛普利加氯沙坦干预组、安慰剂喂养组。5只同系魏凯氏(WKY)大鼠做对照组。8周后取尿检测大鼠24h尿蛋白(Upro)和n-乙酰β-d-氨基葡萄糖苷酶(NAG),取脂肪组织中血管壁用免疫组化法检测脂联素的表达。结果:SHR大鼠脂肪血管壁脂联素表达较正常对照的WKY大鼠降低,Upro和NAG酶水平升高(P均<0.01),福辛普利组和氯沙坦组与SHR组比较血压降低(P<0.01),Upro和NAG酶水平降低(P均<0.01),血管壁脂联素表达上调(P<0.01)。结论:(1)SHR大鼠Upro和NAG酶水平升高,脂肪组织血管壁脂联素表达下调;(2)福辛普利和氯沙坦可以提高SHR大鼠脂肪组织血管壁脂联素表达,降低Upro和NAG酶水平。Objective： To observe adiponectin expression in vascular walls of spontaneously hypertensive rats （SHR）and the effects of fosinopril and lorsartan on it. Methods： Twenty two weeks old male SHR were randomly divided into four groups： Model （SHR） group, fosinopril （Fos） group, losartan （Los） group and fosinopril＋losartan （Fos＋ Los） group; five 22--week old male Wistar-kyoto rats （WKY） were regard as the control group. After 8 weeks, 24 h urinary protein （Upro）, urinary n--acetyl--β--d-- glucosaminidase （NAGase） were detected; the expression of adiponectin in adipose tissue of vascular walls were tested by immunohistochemistry. Results： As compared to WKY group, 24h Upro content and urinary NAGase increased, adiponectin expression in adipose tissue of vascular wails was down-regulated in SHR groups （P〈0.01 all） ; as compared to SHR group, blood pressure was reduced, 24h Upro content and urinary NAGase decreased, adiponectin expression was up-regulated in Fos, Los and Fos＋Los group （P〈0.01 all）. Conclusion： Urinary protein content and urinary NAGase increased, adiponectin expression in adipose tissue of vascular walls is down-regulated in SHR group; fosinopril and/or lorsartan can decrease urinary protein content and urinary NAGase, up-regulate adiponectin expression in adipose tissue of vascular walls of SHR.

关 键 词：大鼠 脂联素 福辛普利 氯沙坦 

分 类 号：R541.75[医药卫生—心血管疾病]