卵泡膜细胞的胰岛素抵抗对其雄激素合成的影响以及胰岛素增敏剂的调控机制  

Direct Effect of Insulin Resistance on Androgen Excess within Porcine Ovarian Thecal Cells

作　　者：高磊[1] 李威[1] 吴效科[1] 侯丽辉[1] J.F.STRAUSS 匡海学[3] 

机构地区：[1]黑龙江中医药大学附属第一医院妇产科,哈尔滨150040 [2]弗吉尼亚联邦大学妇产科学系 [3]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《科技导报》2008年第19期78-83,共6页Science & Technology Review

基　　金：国家自然科学基金项目(30572404);黑龙江省自然科学基金重点项目(ZJY0506-01)

摘　　要：为探索卵泡膜细胞人工诱导胰岛素抵抗后其雄激素合成的变化,以及胰岛素增敏剂曲格列酮、二甲双胍对胰岛素抵抗卵泡膜细胞的调控作用,采用猪卵巢内卵泡膜细胞进行体外培养,地塞米松诱导,构建胰岛素抵抗的细胞模型,用曲格列酮和二甲双胍分别处理模型细胞,检测培养液中葡萄糖和激素水平的变化以及胰岛素信号分子和雄激素合成关键酶的mRNA表达变化。结果表明:卵巢内卵泡膜细胞发生胰岛素抵抗后能明显增进其雄激素合成能力;曲格列酮、二甲双胍均能降低胰岛素抵抗的卵泡膜细胞的糖代谢异常和雄激素水平。To study the direct effect of insulin resistance on androgen excess within thecal cells and the partial improvement by using insulin sensitizers, namely Troglitazone （Tro） and metoformin （Met）, ovarian theca cells from porcine follicles were isolated and cultured in vitro. Insulin resistance was induced in theca cells by Dexamethasone （Dex） treatment, which were further treated by Tro, Met. We determined the level of the medium glucose and testosterone, as well as the cellular expression of key insulin signal and androgen synthesis molecules mRNA or protein expression. The results show that： （1）Insulin resistance induced by Dex could directly and greatly increase androgenic potentials within the theeal cells from porcine ovaries, suggesting the involvement of this ovarian metabolic phenotype in the pathogenesis of androgen excess in PCOS; （2）The beneficial effects of two sensitizing agents on reproduction may result from the alleviation of insulin resistance and androgen synthesis commonly found in PCOS treatment, indicating a local role within polycystic ovaries.

关 键 词：胰岛素抵抗 卵泡膜细胞 胰岛素增敏剂 多囊卵巢综合征 

分 类 号：R711.75[医药卫生—妇产科学] R587[医药卫生—临床医学]