心房钠尿肽合成细胞在大鼠胃粘膜的超微结构定位及与微血管密度的关系  

Ultrastructural localization of ANP-synthesizing cells and relationship between ANP-synthesizing cells and microvessel density in rat stomach

作　　者：潘理会[1] 韩晨光[2] 李春辉[1] 

机构地区：[1]承德医学院,河北承德067000 [2]武警医学院附属医院医教部,天津300162

出　　处：《武警医学院学报》2008年第11期950-953,F0003,共5页Acta Academiae Medicinae CPAPF

基　　金：河北省承德市科技攻关计划项目(200721047)

摘　　要：【目的】研究心房钠尿肽合成细胞在大鼠胃粘膜的超微结构定位及与微血管密度的关系。【方法】采用包埋后免疫胶体金标记电镜技术对大鼠胃粘膜ANP合成细胞进行超微结构定位,采用藏红花-甲苯胺蓝染色对ANP合成细胞进行标记,采用单宁酸-氧化铁染色和扫描电镜技术对大鼠胃粘膜的微血管密度进行测定。【结果】胃粘膜内肠嗜铬细胞有ANP阳性表达;大鼠胃贲门区ANP合成细胞与微血管密度呈正相关(r=0.53,P<0.05,n=18)。【结论】胃粘膜的肠嗜铬细胞系ANP合成细胞,大鼠胃贲门区ANP合成细胞的分布密度与微血管密度之间存在相关性。[ Objective] To investigate ultrastructural localization of ANP-synthesizing cells and relationship between ANP-synthesizing cells and microvessel density in rat stomach. [ Methods] Ultrastructural localization of ANP-synthesizing cells in rat stomach was analyzed by postembedding immunoelectron microscopy. The area density of the distribution of ANP-synthesizing cells was calculated by saffron solution and toluidine solution staining. Microvessel of rat stomach was revealed distinctively by TA-Fe staining method and scanning electron microscope. [ Results] Expression of ANP was found in enterochrochromaffin cells （EC） of rat stomach. There was a positive significant interrelationship between positive rate of ANP-synthesizng cells and microvessel density in arttral mucosa of rats （ r = 0.53, P 〈 0.05, n = 18）. [Conclusions] Enterochromaffin cells （EC） can synthesize ANP. There was a positive significant interrelationship between positive rate of ANP-synthesizng cells and of microvessel density in antral mucosa of rats.

关 键 词：心房钠尿肽合成细胞 胃肠嗜铬细胞 微血管密度 定位 胃黏膜 大鼠 

分 类 号：Q24[生物学—细胞生物学]