反相高效液相色谱荧光检测法测定血浆中阿霉素的浓度  

Determination of Doxorubicin in Plasma by RP-HPLC with Fluorescence Detector

作　　者：吕兴萍[1] 童姗姗[2] 

机构地区：[1]江苏农林职业技术学院,江苏句容212400 [2]江苏大学药学院,江苏镇江212001

出　　处：《安徽科技学院学报》2008年第5期31-35,共5页Journal of Anhui Science and Technology University

摘　　要：目的:建立一种RP-HPLC检测盐酸阿霉素在小鼠体内药物浓度的方法。方法:血浆中样品以氯仿-乙醇(10:1)混合液提取,NovapakC18柱为分析柱,甲醇-乙腈-0.02mol/L磷酸二氢铵-冰醋酸(52:5:43:0.6V/V)为流动相,Ex=246nm、Em=555nm为检测波长,以α-萘酚为内标。结果:0.0102-4.080μg/mL范围内线性关系良好(r=0.995),日内RSD<10.0%,最低检测浓度为5ng/mL,平均回收率为97.22%。结论:该方法灵敏、简便,符合临床药浓监测及药代动力学研究的要求。This research is to establish an analytical method for the determination of doxorubicin in plasma by reversed phase HPLC with fluorescence detector, with the method that Doxorubicin in plasma was extracted by trichloromethane - methanol （ 10 ： 1 ）. A Reverse - phase C 18 column （ Novapak, Waters, 3.9 ＊ 150mm） with a particle size of 4μm was used. A solution of methanol- acetonitrile -0.02mol/L NH4H2PO4 -acetic acid（52 ： 5 ： 43 ： 0.6）was used as the mobile phase. The detection was completed at 246nm as Ex wavelength and 555nm as Em wavelength, α- naphthol was used as the internal standard. The linear range, the detection limit, the av- erage - recovery and RSD obtained were 0.0102 -4.080μg/mL（ r = 0. 995 ）, 5ng/mL,97.22% and 5.1% - 11.5% （n = 5 ）respectively. The method is simple with high sensitivity and good precision and has proved to be suitable for pharmacokinetic studies of doxorubicin.

关 键 词：高效液相色谱法 阿霉素 血浆 

分 类 号：O657.7[理学—分析化学]