虾夷扇贝肌动蛋白基因cDNA序列克隆与分析  

Cloning and Sequence Analysis of Ful-length cDNA of Actin Gene from Japanese Scallop(Patinopecten yessoensis)

作　　者：刘卫东[1,2] 赫崇波[2] 刘彤 鲍相渤[2] 虞星炬[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]辽宁省海洋水产科学研究院,辽宁省应用海洋生物技术开放实验室,辽宁省海洋水产分子生物学重点实验室,辽宁大连116023 [3]大连市水产技术推广总站,辽宁大连116023

出　　处：《水产科学》2008年第10期519-522,共4页Fisheries Science

基　　金：国家留学人员择优资助项目(LXZZ2005001);大连市青年科学基金资助项目(2005J22JH047)

摘　　要：采用RT-PCR和RACE法从虾夷扇贝闭壳肌中分离和克隆了肌动蛋白基因cDNA全长序列。肌动蛋白基因cDNA全长1775 bp(不包括poly A),5′端非编码区94 bp,3′端非翻译区551 bp,阅读框1131 bp,编码376个氨基酸。在基因组DNA中,该基因被一个内含子分为两段,内含子位于第42和第43个氨基酸之间,长度为2041 bp。系统发育分析显示该肌动蛋白属于α类型。The full length cDNA of actin gene of Japanese scallop （ Patinopecten yessoensis） adductor muscle was cloned and sequenced using RT-PCR and RACE methods. The cDNA was 1775 bp with 94 bp of 5UTR, 551 bp of 3UTR, and 1131 bp of ORF, which encodes 376 amino acids. One intron （2041 bp） was detected between codons 41 and codons 42 in the gene. Sequence analysis revealed that the nucleotide sequence identity of this gene between Japanese scallop and other animals was from 85% to 96%, and the similarity of amino acid sequence was from 93% to 98%. The phylogenetic analysis constructed based on the amino acid sequences suggesting that this actin be a kind of α actin.

关 键 词：虾夷扇贝 肌动蛋白 RACE 克隆 

分 类 号：S968.313[农业科学—水产养殖]