脓毒症大鼠肾脏线粒体膜电位变化和超微结构损伤的研究  

Experimental study on mitochondrial membrane potential and ultrastructure injuries of kidney in septic rats.

作　　者：陈飞燕[1] 曾其毅[1] 赵明奇[1] 杨文敏[1] 连广琬[1] 

机构地区：[1]广州医学院附属广州市儿童医院,510120

出　　处：《中国小儿急救医学》2008年第5期438-441,共4页Chinese Pediatric Emergency Medicine

基　　金：广州市医药卫生科技重点项目（2007-ZDi-09）

摘　　要：目的探讨脓毒症时大鼠肾脏线粒体的损伤情况。方法采用腹腔注射内毒素（LPS）建立脓毒症大鼠模型。30只SD大鼠随机分为对照组、6h脓毒症组和24h脓毒症组。分别检测各组大鼠血清肌酐（Cr）、尿素氮（BUN）水平；电镜观察肾脏线粒体形态学变化；运用流式细胞术检测分离肾脏线粒体的肿胀程度和线粒体膜电位，用前向角与侧向角的平均荧光强度比值（FSC／SSC）反映线粒体肿胀程度，用二通道和一通道平均荧光强度比值（FL2／FL1）确定线粒体膜电位。结果脓毒症大鼠的血清Cr、BUN上升明显高于对照组（P〈0．05）；肾脏线粒体肿胀程度（FSC／SSC）在24h脓毒症组中明显增加（对照组：0．55±0．10；6h脓毒症组：0．58±0．10；24h脓毒症组：0．66±0．12，P〈0．05）；肾脏线粒体膜电位（FL2／FL1）在6h脓毒症和24h脓毒症组中均明显下降（对照组：0．77±0．26；6h脓毒症组：0．32±0．19；24h脓毒症组：0．30±0．17，P〈0．01），其下降趋势与大鼠血清Cr变化呈负相关（r=-0．510，P=0．004）；肾脏线粒体超微结构在24h脓毒症组中损伤明显。结论脓毒症早期肾脏功能和线粒体膜电位存在损伤；但线粒体超微结构损伤在脓毒症早期表现轻微，晚期损伤明显。Objective To study the mitochondrial membrane potential and ultrastructure injuries of kidney in septic rats. Methods Thirty SD rats were randomly divided into three groups：Saline control group, 6 h LPS group and 24 h LPS group. The rats in LPS groups received 10 mg/kg lipopolysaccharide by intraperitoneal injection. The levels of serum Cr and BUN were detected. The swelling （FSC/SSC） and the membrane potential （FL2/FL1） of isolated renal mitochondrion were tested by flow cytometry. The morphologic change of mitochondria in kidney was observed by electronic microscopy. Results Compared with control group, serum Cr and BUN increased in septic rats. Compared with control group, swelling of the mitochondrion significantly increased in 24 h LPS group （Control： 0.55 ± 0.10 ; 6 h LPS： 0.58 ± 0.10; 24 h LPS： 0.66 ± 0.12, P 〈 0.05 ）. Renal mitochondrial membrane potential significantly decreased in 6 h LPS group and 24 h LPS group （Control： 0.77 ± 0.26;6 h LPS：0.32 ± 0.19;24 h LPS：0.30 ± 0.17, P〈 0.01）. The mitochondrial membrane potential in kidney were inversely correlated with serum Cr level（ r = - 0. 510, P = 0.004 ）. The injuries of mitochondrial ultrastructure increased in 24 h LPS group. Conclusion The injury of the renal mitochondrial membrane potential has occurred in early stage of sepsis before obvious ultrastructure damage of the renal mitochondrion in septic rats.

关 键 词：脓毒症 线粒体 肾脏 SD大鼠 

分 类 号：R459.7[医药卫生—急诊医学]