卡托普利对高氧致新生大鼠肺纤维化的保护作用  

Protect effects of Captoprii on neonatal Wistar rats with lung fibrosis induced by hyperoxia

作　　者：李玖军[1] 薛辛东[1] 王书堂 

机构地区：[1]中国医科大学附属盛京医院儿科,沈阳110004 [2]河北省邯郸市成安县医院儿科,056700

出　　处：《中国小儿急救医学》2008年第5期454-457,共4页Chinese Pediatric Emergency Medicine

基　　金：国家自然科学基金资助项目（30440056）

摘　　要：目的观察卡托普利对高氧致新生大鼠肺组织纤维化的保护作用。方法足月新生Wistar大鼠240只，随机分为模型组、空气对照组、治疗组和盐水对照组，每组各60只。模型组、盐水对照组和治疗组将足月新生Wistar大鼠（连同母鼠）生后即置于氧舱内持续吸入高浓度氧（FiO2：0．9）21d造成高氧肺损伤模型。空气对照组吸入空气；治疗组于生后7d每天经胃管灌服卡托普利30mg／（kg·d）（用生理盐水配成5．4mg／ml混悬液），盐水对照组每天经胃管灌服等量生理盐水。每组分别于实验开始后的第1、3、7、14、21天随机选取6只麻醉后处死。肺组织采用酶联免疫吸附法（ELISA）测定Ⅲ型胶原的含量，用放射免疫法测定血管紧张素Ⅱ（AngⅡ）的含量。用逆转录聚合酶链反应（RT-PCR）检测肺组织AngⅡ、Ⅲ型胶原mRNA表达的动态变化，同时观察肺组织形态学变化。结果模型组及盐水对照组肺组织AngⅡ、Ⅲ型胶原含量及mRNA表达第14天明显升高，除盐水对照组的AngⅡmRNA表达升高不明显外，与空气对照组比较差异均有显著性（P〈0．05）；两组各项指标在第21天达到高峰，模型组肺组织AngⅡ、Ⅲ型胶原含量分别为（838．22±197．75）、（104．21±43．37）ng／mg，与空气对照组比较差异有显著性（P均〈0．01）；盐水对照组肺组织AngⅡ、Ⅲ型胶原含量分别为（759．97±60．81）、（128．69±54．74）ng／mg，与空气对照组比较差异有显著性（P均〈0．05）。治疗组第21天AngⅡ、Ⅲ型胶原含量分别为（554．52±59．32）、（39．90±13．45）ng／mg，其mRNA表达分别为1．50±0．84、1．13±0．55，均明显低于模型组及盐水对照组（P均〈0．05），但仍高于空气对照组（P均〈0．05）。肺组织形态学改变：空气对照组为正常肺组织，模型组、盐水对照组第1天同空气对照组，第3～7天肺泡壁毛细血管�Objective To observe the dynamic changes and the effects of Captopril on interstitial fibrosis in lung tissue of neonatal rats with lung fibrosis induced by hyperoxia. Methods Two hundred and forty neonatal Wistar rats were randomly assigned into model group, air control group, normal saline control and Captopril-treated group （n = 60 each）. The air control group was exposed to room air （FiO2 = 0.21）, and the rest three groups were continuously exposed to hyperoxia （FiO2 = 0.9） for 21 days. During the exposure, the Captopril-treated group received Captopril [30 mg/（kg· d）] by intragastric administration, and the normal saline control group was administrated with normal saline instead, the model group did not receive any treatment. On the i st, 3 rd, 7 th, 14 th and 21 st day of exposure, the subjects were sacrificed. And then, the protein levels of collagen Ⅲ （Co-Ⅲ） were measured by enzyme-linked immunosorbent assay and angiotensin Ⅱ （Ang Ⅱ ）by radio-immunity technique, and the mRNA expression of Ang Ⅱ, Co-Ⅲ was measured by RT-polymerase chain reaction. The changes of lung histomorphology were observed. Results On the 14 th day, The Ang Ⅱ, Co- Ⅲ protein levels and their mRNA expression of model group and normal saline control group increased significantly as compared to the air control group （ P 〈 0.05 ）, except the Ang Ⅱ mRNA expression of normal saline control group. The Ang Ⅱ and Co-Ⅲ protein levels of model group was （838.22 ± 197.75） and （ 104.21 ± 43.37） ng/mg respectively, and normal saline control group was （759.97 ± 60.81 ） and （ 128.69 ± 54.74） ng/mg respectively on the 21 st day, their mRNA expression of two groups also increased to the peak on the 21 st day（P 〈 0.05 ）. The Ang Ⅱ and Co-Ⅲ protein levels of Captopril-treated group was （554.52 ± 59.32） and （39.90 ± 13.45） ng/mg on the 21 st day respectively, their mRNA expression was （1.50 ± 0.84） and （ 1.13 ± 0.55 ） respectively, and decreased signific

关 键 词：卡托普利 高浓度氧 肺纤维化 

分 类 号：R285.5[医药卫生—中药学]