基质金属蛋白酶-9及其抑制剂-1在子宫腺肌病子宫内膜-肌层界面的表达  

The Expression of MMP-9 and TIMP-1 in the Endometrial-myometrial Interface of Adenomyosis

作　　者：刘素巧[1] 高英芳[2] 吴小华 申莉瑛 董晓瑜[1] 陈靖[1] 

机构地区：[1]河北省石家庄市第二医院妇产科,石家庄050051 [2]河北省石家庄市第四医院,石家庄050011 [3]河北省石家庄市白求恩国际和平医院妇产科,石家庄050082

出　　处：《中国医药导刊》2008年第6期896-897,共2页Chinese Journal of Medicinal Guide

基　　金：河北省卫生厅医学研究重点课题(No.06126)

摘　　要：目的:探讨子宫腺肌病患者在位子宫内膜、子宫内膜-肌层界面(endometrial-myometrial interface,EMI)基质金属蛋白酶-9 (MMP-9)及其抑制物-1(TIMP-1)的表达及其生物学意义。方法:采用免疫组化SP方法对40例子宫腺肌病的子宫内膜(含部分肌层)和40例子宫肌瘤的子宫内膜(含部分肌层)的组织进行分析。结果:腺肌病组MMP-9的表达强度EMI处显著高于远肌层(P<0.05)。EMI处MMP-9表达,腺肌病组显著高于对照组(P<0.05)。EMI处TIMP-1在增生期和分泌期的表达,腺肌病组明显低于对照组(P<0.05)。TIMP-1在EMI和远肌层的表达比较无显著差异(P>0.05)。结论:子宫腺肌病组EMI处的内膜腺体中MMP-9的高表达,可使子宫内膜的侵蚀能力增强,可能参与子宫腺肌病的发生和发展。Objective： To study the expression of matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metaUoproteinase-1（TIMP- 1）in uterine endometria （functional layer） and the endometrial-myometrial interface （EMI） of adenomyosis and their biological significance. Method： SP immunohistochemistry staining was used to detect the expression of MMP-9 and TIMP-1 of EMI and uterine endometria（the functional layer）among 40 cases with adenomyosis and 40 cases with leiomyoma. Results： The expression of MMP-9 in EMI of adenomyosis group was stronger than that in uterine endometria of fimctional layer （P〈0.05）.The staining intensity of MMP-9 in EMI of study group was much stronger than that of control group （P〈0.05）. The expression of TIMP-t in EMI of adenomyosis group was lower than that of control group（P〈0,05）.The expression of TIMP-1 was no difference between EMI and uterine endometria （P〉0.05）.Conclusions： Higher expression of MMP-9 in EMI might enhance the endometrial invasive ability. It may be related to the pathogenesis of adenomyosis.

关 键 词：子宫内膜-肌层界面 基质金属蛋白酶-9 基质金属蛋白酶抑制剂-1 子宫腺肌病 免疫组化 

分 类 号：R711.71[医药卫生—妇产科学]