HPLC法测定醒脑库克亚片中芦荟苷的含量  

Determination of Barbalion in XingnaoKukeya Tablet by HPLC

作　　者：谢玲[1] 潘苇芩 李学强[1] 李晓惠 敬静[4] 

机构地区：[1]新疆军区联勤部药品仪器检验所,新疆乌鲁木齐830063 [2]新疆自治区药检所,新疆乌鲁木齐830002 [3]乌鲁木齐市综合仓库,新疆乌鲁木齐830002 [4]新疆农业大学042本科班实习生

出　　处：《解放军药学学报》2008年第5期444-445,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立HPLC法测定维吾尔药醒脑库克亚片中芦荟苷含量的方法。方法色谱柱为Shimadzu ODS VP（4.6mm×250mm，5μm）；流动相为乙腈-水（25：75）；流速为1.0ml／min；检测波长为360nm；柱温：30℃。结果芦荟苷进样量在0.238-2.38℃g范围内呈良好的线性关系（r=0．9999），平均加样回收率为98．80％，RSD为2．0％（n=5）。结论本方法操作简单、结果准确、重复性好，可用于该制剂的质量控制。Aim To develop a method for determination of barbalion in Xingnaokukeya tablets of Uygur medicine. Methods HPLC with Shimadzu ODS VP （4.6mm×250mm,5μm）column was used. The mobile phase consisted of aceto-nitrile-water （25： 75） ,with a flow rate of 1.0ml/min and detection wavelength at 360nm and under 30℃. Results The linear range was 0.238-2.38μg（ r =0.9999）. The average recovery （ n = 9）was 98.80 % and RSD was 2.03%. Conclusion The method is simple ,accurate,sensitive and could be used for quality control of the preparation.

关 键 词：醒脑库克亚片 芦荟苷 HPLC 

分 类 号：R917.2[医药卫生—药物分析学]