甲醛致雄性大鼠脑和睾丸细胞DNA-蛋白质交联的研究  

Study on DNA-protein Crosslinks in Brain and Testicle Cells Induced by Formaldehyde in Male Mice

作　　者：王红蕾[1] 柯砚[1] 肖琳[1] 付聪[1] 魏晨曦[1] 王昆[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,湖北武汉430079

出　　处：《环境与健康杂志》2008年第10期853-855,共3页Journal of Environment and Health

基　　金：国家自然科学基金面上项目(30570799)

摘　　要：目的探讨气态甲醛致雄性大鼠脑细胞和睾丸细胞 DNA-蛋白质交联(DNA-protein crosslinks,DPC)作用。方法将24只 SPF 级 Wistar 雄性大鼠随机分为4组,每组6只,分别为0.5、1.0、3.0 mg/m^3气态甲醛染毒组和阴性对照组。连续动态吸入染毒72 h 后,应用 KCl-SDS 沉淀法检测大鼠脑和睾丸组织 DNA-蛋白质交联的含量。结果与阴性对照组比较,0.5mg/m^3气态甲醛染毒组大鼠脑和睾丸组织的 DPC 系数差异无统计学意义(P>0.05),随着甲醛浓度的增加,DPC 系数逐渐升高。与阴性对照组比较,1.0、3.0mg/m^3气态甲醛染毒组大鼠脑和睾丸组织 DPC 系数升高,差异有统计学意义(P<0.01)。结论低浓度(0.5 mg/m^3)的气态甲醛不能引起大鼠脑和睾丸组织产生 DPC 效应,而较高浓度(1.0、3.0mg/m^3)的气态甲醛可明显诱导其产生 DPC 效应,造成严重的 DNA 损伤。Objective To explore the DNA-protein crosslinks （DPC） effect induced by gaseous formaldehyde （FA） in the brain and testicle cells of Wistar male rats. Methods Wistar male rats were randomly divided into four groups, 6 in each and exposed to gaseous FA at doses of 0（control group）, 0.5, 1.0 and 3.0 mg/m^3 for 72 consecutive hours. The brain and testicle cells were used as the experimental materials. The KCl-SDS assay was applied to determine the amount of DNA-protain crosslinks. Results Compared with the control group, no significant change in DPC amount was observed at the low concentrations exposure group （0.5 mg/m^3, P〉0.05）, but as formaldehyde concentration increased, the coefficient of DPC also increased gradually. Higher concentration exposure（1.0, 3.0 mg/m^3） resulted in significant elevation of DPC amount compared with the control group（P〈0.01）. Conclusion At the low concentration, gaseous formaldehyde exposure can not cause DPC, but DPC can be induced after higher concentrations exposure, then serious DNA danmge will follow.

关 键 词：甲醛 喃细胞 睾丸细胞 DNA损伤 DNA-蛋白质交联 

分 类 号：R994.6[医药卫生—毒理学]