云母颗粒对无水乙醇诱导大鼠胃黏膜损伤的影响及机理探讨  

Discussion on Mechanism and Effect of Mica Granules on Rats' Stomach Mucosa Injury Induced by Non-water Ethanol

作　　者：钦丹萍[1] 吕海丽[1] 邵国民[1] 任永葆[1] 韩建新[1] 邱添[1] 黄一鸣[1] 

机构地区：[1]浙江中医药大学第一临床医学院,杭州310053

出　　处：《浙江中医药大学学报》2008年第5期595-597,共3页Journal of Zhejiang Chinese Medical University

基　　金：浙江省教育厅立项课题(No:20030878)~~

摘　　要：[目的]研究云母颗粒对无水乙醇诱导大鼠胃黏膜损伤的保护作用及其机理。[方法]将48只健康雄性SD大鼠随机分为云母颗粒高、中、低剂量组,思密达组,模型对照组,空白对照组6组。分别将云母颗粒和思密达预先灌胃于相应各组大鼠,3h后以1ml/100g无水乙醇灌胃致胃黏膜损伤,45min后下腔静脉取适量血,处死取胃,然后分别检测各组的胃黏膜损伤指数、血清和胃黏膜组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)含量,并观察病理学变化,以评价胃黏膜损伤程度及药物的作用。[结果]云母各剂量组中SOD、GSH-Px含量均显著高于模型对照组,而MDA含量则明显低于模型对照组(P<0.05)。[结论]云母颗粒对无水乙醇诱导的大鼠胃黏膜损伤具有预防与保护作用,其保护作用是通过增加大鼠血清和胃黏膜组织中SOD、GSH-Px含量并减少MDA的生成来实现的,即云母颗粒具有抗脂质过氧化作用。[Objective] To study the protection and mechanism of mica granules to rats stomach mucosa injury caused by non-water ethanol. [Method] Randomly divide 48 healthy SD rats into high-, middle-and low-dosed groups of mica granules, Simida group, model control group and blank control group as well,i, e. 6 groups. After fasting for 24h, separately make stomach perfusion of mica granules and Simida to all rats in advance;after 3h,make stomach perfusion of non-water ethanol 1ml/100g for stomach mucosa injury;45m later,take proper blood of lower vena cava, sacrifice them for stomaeh, then respectively test all mucosa injury index, SOD,MDA, and GSH-Px content in serum and mucosa,observe pathological changes to evaluate mucosa injury degree and medical function. [Result] In mica groups, the SOD and GSH-Px were markedly higher than that in model control group, but MDA was lower than later（P〈0.05）. [Conclusion] Mica granules have prevention and protection to rats gastric mueosa injury caused by non- water ethanol,which is produced from mica granules＇ increasing SOD, GSH Px content and decreasing MDA in serum and gastric mucosa,i, e. mica granules have function of anti-lipid peroxidation.

关 键 词：云母颗粒 无水乙醇 损伤指数 损伤程度 超氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]