绵羊尿鸟苷素基因的克隆与序列分析  

Molecular Cloning and Sequence Analysis of Ovine Uroguanylin

作　　者：乔新安[1] 王艳玲[1] 杨国宇[1] 朱彦彩[2] 范沛[1] 林茂旺[1] 

机构地区：[1]河南农业大学动物生理生化实验室,河南郑州450002 [2]河南科技学院细胞工程重点实验室,河南新乡453003

出　　处：《河南科技学院学报》2008年第1期54-56,共3页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：河南省重点科技攻关项目(0523010500)

摘　　要：克隆并分析了绵羊尿鸟苷素(uroguanylin)基因。根据同源序列克隆原理设计一对克隆引物,对绵羊十二指肠黏膜组织提取的总RNA进行RT-PCR,将PCR产物克隆、测序。绵羊尿鸟苷素基因与人、小鼠和猪的同源性分别为83%,77%和88%,推测的氨基酸构成一个模域。克隆了绵羊尿鸟苷素基因片断,为进一步研究绵羊尿鸟苷素基因的生物学功能提供了理论基础。To clone and analysis the ovine uroguanylin. According to the cloning principle of homologous sequence we designed the cloning primers. Total RNA extracted from mucosal tissue of ovine duodenum was amplified by RT - PCR, then the PCR product was cloned and sequenced. The results show that ovine uroguanylin shared 83% , 77% and 88% homology with that of human, mouse and pig. The Pfam domain is formed by the predicted amino acid sequence. The ovine urouanylin was successfully cloned to establish theoretical basis for its further biological function.

关 键 词：绵羊 尿鸟苷素 克隆 表达 

分 类 号：S811.3[农业科学—畜牧学] Q789[农业科学—畜牧兽医]