铅抑制NF-κB、Bcl-2蛋白表达诱导人肾小管上皮细胞凋亡  

Effect of Lead on Apoptosis and Expression of NF-κB and Bcl-2 in Human Proximal Tubular Epithelial Cell Line HK-2

作　　者：周桂凤[1] 蒋云生[1] 胡白英[1] 杨波[1] 乔玉峰[1] 刘栋[1] 

机构地区：[1]中南大学湘雅二医院肾内科,湖南长沙410011

出　　处：《实用预防医学》2008年第5期1330-1332,共3页Practical Preventive Medicine

基　　金：湖南省自然科学基金资助项目(05JJ40115)

摘　　要：目的研究醋酸铅对人肾小管上皮细胞系HK-2细胞NFκB、Bcl-2表达的影响及与细胞凋亡的关系。方法体外培养HK-2细胞分为0、2.5、5、10、20μmol/L醋酸铅组,分别染毒72h,应用间接免疫荧光-流式细胞术检测HK-2细胞NF-κB、Bcl-2蛋白表达的改变,用Annexin-V/PI双染法检测细胞凋亡。结果随着染铅剂量增加HK-2细胞NF-κB、Bcl-2蛋白表达逐步降低,NF-κB与Bcl-2降低显著相关(r=0.71,P<0.01);细胞凋亡率随着铅剂量增加而增加,在5、10、20μmol/L铅组与对照组比较差别有统计学意义(P<0.01);细胞凋亡率与NF-κB、Bcl-2蛋白表达呈明显负相关(r=-0.625,P<0.01;r=-0.709,P<0.01)。结论醋酸铅能下调HK-2细胞NF-κB、Bcl-2基因的表达,这可能是其诱导HK-2细胞凋亡的途径之一。Objective To investigate whether lead acetate （Pb） can induce apoPtosis in human proximal tubular epithelial cell line HK- 2, and to explore the molecular mechanism involved. Methods HK 2 cells were cultured in RPMI 1640 medium containing different concentrations of lead acetate （0, 2.5, 5, 10, 20μmol/L） for 72h. The flow cytometry and Annexin - V/PI double staining were employed to investigate the effects of Pb on the HK 2 cells and to explore possible related mo- lecular mechanisms. Results Pb elevated apoptosis of HK- 2 cells. The rates of apoptosis at 5, 10, and 20μmol/L of Pb were significantly higher than those of control group （P〈 0.01）. Pb also decreased NF-κB and Bcl 2 protein expression, significant differences of NF-κB protein expression was found between 0μmol/L and 10μmol/L. Correlation analysis showed that the rates of apoptosis were inversely correlated with the expression of NF-κB and Bcl- 2 （r = -0. 625, P〈0.01; r = - 0. 709, P〈 0.01）. Conclusions Pb induces apoptosis by inhibiting NF -κ and Bcl - 2, expression in human proximal tubular epithelial cell line HK- 2.

关 键 词：醋酸铅 肾小管上皮细胞 NF-ΚB Bcl-2 凋亡 

分 类 号：R994.1[医药卫生—毒理学]