牛胎儿皮肤成纤维细胞体外培养及其特性研究  

Studies on the in Vitro Culture and the Characteristics of Bovine Fetus Skin Fibroblasts

作　　者：周艳喜[1] 曲世磊[1] 李鹏[1] 李燕[1] 刘春霞[1] 

机构地区：[1]内蒙古农业大学生物工程学院,内蒙古呼和浩特010018

出　　处：《畜牧与饲料科学》2008年第5期24-26,共3页Animal Husbandry and Feed Science

摘　　要：该试验研究了牛胎儿皮肤成纤维细胞的分离、培养、纯化方法及其生长特征。牛胎儿皮肤细胞贴壁传代2～4次,可成功获得均一稳定的成纤维细胞群体。取传至4代的牛胎儿皮肤成纤维细胞进行细胞计数,并绘制其生长曲线。The study focused on the dissociation, culture, purification and characteristics of bovine fetus skin fibroblasts. Bovine fetus skin fibroblasts had been ohtained by 2-4 times subcultures in vitro. The growth curve had been drawn by cell counting. The 5th, 6th, 7th and 8th passage bovine fetus skin fihrohlasts were manipulated through the technologies of chromosome preparation and microscopic examine of the chromosome change.

关 键 词：牛 成纤维细胞 体外培养 

分 类 号：Q813.11[生物学—生物工程]