TH和ChAT阳性神经元在大鼠面神经核团中的分布及其意义  

The distribution of TH and ChAT positive neurons in the facial nucleus of rats and its implication

作　　者：赵真[1] 米文娟[1] 刘顺利[1] 邱建华[1] 

机构地区：[1]第四军医大学西京医院耳鼻咽喉-头颈外科,西安710032

出　　处：《中华耳科学杂志》2008年第3期273-277,共5页Chinese Journal of Otology

摘　　要：目的研究大鼠面神经核(facial nucleus)中酪氨酸羟化酶(tyrosine hydroxylase,TH)、胆碱乙酰转移酶(choline acetyl transferase,ChAT)免疫反应阳性神经元胞体及神经纤维的分布特点并探讨其意义。方法采用免疫组织化学及免疫荧光双标技术检测TH和ChAT免疫阳性神经元在大鼠面神经核中的分布。结果TH阳性神经元胞体主要分布在腹侧中间亚核(ventral intermediate subnucleus)、腹内侧亚核(ventromedial subnucleus)、外侧亚核(lateral subnucleus)。ChAT阳性神经元胞体大量分布在面神经核团中,主要分布在腹内侧亚核、背内侧亚核(dorsomedial subnucleus)、背外侧亚核(dorsolateral subnucleus)等面神经亚核。在面神经核腹内侧亚核及中间亚核尚可见大量TH阳性神经终末,包绕TH、ChAT双标神经元胞体。结论TH与ChAT在面神经核内存在神经调质共存现象。Objective To study the distribution of neurons and fibers which are positive to tyrosine hydroxylase （TH）） and choline acetyl transferase（ChAT） in the facial nucleus of rats and explore its implication. Methods Both immunohistochemistry and immunofluorescence are used to document the TH and ChAT positive neurons. Results TH positive neurons mainly located in the ventral intermediate subnucleus（VI）, ventromedial subnucleus（VM）, lateral subnucleus（L）, while ChAT positive neurons in the ventromedial subnucleus（VM）, dorsomedial subnucleus（DM） and dorsolateral subnucleus（DL）. And in VM and VI we also found plenty of TH positive nervous endings, which surrounded the neurons positive to both TH and ChAT. Conclusion There are coexistence of TH and ChAT as neuromodulators in the facial nucleus of rats.

关 键 词：酪氨酸羟化酶 胆碱乙酰转移酶 面神经核 神经构筑 

分 类 号：R332.86[医药卫生—人体生理学]