生物活性玻璃/胶原蛋白/磷酸丝氨酸复合支架材料的制备及其细胞相容性探讨  

The Preparation of Bioglass/Collagen/Phosphoserine Biomemetic Composite Scaffold and a Study on Its Cytocompatibility

作　　者：陈晓峰[1,2] 李向军[1] 王迎军[1,2] 杨春蓉[1] 赵娜如[1,2] 

机构地区：[1]华南理工大学材料学院生物材料研究所,广州510640 [2]华南理工大学特种功能材料教育部重点实验室,广州510640

出　　处：《生物医学工程学杂志》2008年第5期1112-1115,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(50672028;50472054);广东省自然科学基金资助项目(05006587;04205786);国家973项目资助(2005CB623902);广州市科技计划项目资助(2003Z1-D0021)

摘　　要：通过冷冻干燥方法制备出仿生型多孔结构的溶胶-凝胶生物活性玻璃(BG)/胶原蛋白(COL)/磷酸丝氨酸(PS)支架材料,并评价了小鼠胚胎成骨细胞系细胞在BG、BG-COL、BG-COL-PS支架材料上的生长、黏附、增殖情况。MTT法测结果显示,细胞在材料表面贴附、增殖良好,且细胞在BG-COL-PS支架材料生长增殖要明显好于BG-COL、BG支架材料。BG-COL-PS支架材料有可能成为一种新型的骨组织工程支架材料。In the present study, novel biomemetic composite scaffolds using the sol-gd derived bioactive glass（BG）, collagen（COL）, and phosphoserine{PS） were prepared by freeze-drying. MC3T3-E1 were cultivated in vitro, collected and seeded onto the surface of 13G, I^-COL and t^-COL-PS. Cell attachment and proliferation were observed. The cell proliferation was tracked by MTT method 1,3,5 d after seeding. MTT showed that the cells can adhere to and proliferate well on the surface of the scaffolds, and the cell proliferation result of scaffold BG-COL-PS was better than those of scaffolds BG and BG-COL. Therefore, the scaffold BG-COL-PS can be a potential scaffold for tissue engineer.

关 键 词：生物活性玻璃 胶原 磷酸丝氨酸 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程] Q256[医药卫生—基础医学]