聚合物微囊型血液代用品携氧-释氧性能的无损检测方法  

A Nondestructive Method to Measure the Oxygen Binding and Releasing Properties of Biodegradable Polymers Microcapsules Intented for Blood Substitutes

作　　者：章晓兰[1] 袁媛[1] 王凯[1] 单晓茜[1] 赵健[1] 盛燕[1] 刘昌胜[1] 

机构地区：[1]华东理工大学,教育部医用生物材料工程研究中心,上海200237

出　　处：《生物医学工程学杂志》2008年第5期1116-1120,共5页Journal of Biomedical Engineering

基　　金：十五"863"纳米专项基金资助项目(2004AA-302050);上海市科委纳米专项资助项目(0452nm022)

摘　　要：P50是衡量血液代用品携氧-释氧性能的重要指标。本研究借鉴临床脉冲血氧饱和度仪的工作原理,利用近红外光具有强穿透性(能穿透可生物降解聚合物壳材并对包封在壳材内的血红蛋白进行检测),结合氧合血红蛋白和脱氧血红蛋白在近红外光区域有不同的吸收光谱特性,采用自行研制的密封、平衡装置完成对载牛血红蛋白微囊型血液代用品的血氧饱和度和对应氧分压的测定,从而得出氧解离曲线、P50等携氧-释氧指标。该套装置和方法无需破损微囊,过程简单,可实现对聚合物微囊型血液代用品携氧-释氧性能的无损、在位检测。P50 is an important parameter reflecting the binding and releasing oxygen properties of blood substitutes. In this study, based on the strong penetrating property of near infrared light and the mechanism involved in the pulsatile oxygen meter in clinic as well as on the ability for penetrating biodegradable polymers and detecting bovine hemoglobin encapsulated within the microcapsules, we have made an airproof and equilibrium apparatus to measure oxygen saturation and oxygen partial pressure. Subsequently, we have obtained the oxygen dissociation curve and P50 of the microcapsules loaded bovine hemoglobin in the light of oxyHemoglobin and deoxyHemoglobin with different spectrum in the near infrared region. The above-mentioned apparatus and method are not destructive to the microcapsules, and the process is simple and nondestructive. So it is practical to take in-situ measurements of the oxygen binding and releasing property of biodegradable polymer microcapsules intented for the blood substitute.

关 键 词：P50 携氧-释氧 近红外光 载牛血红蛋白微囊 密封平衡装置 无损检测 

分 类 号：R927[医药卫生—药学]