纳秒脉冲用于细胞内电处理的研究进展  

Research Progress of Nanosecond Pulsed Electric Field Applied to Intracellular Electromanipulation

作　　者：姚陈果[1] 莫登斌[1] 孙才新[1] 陈新[1] 熊正爱[2] 

机构地区：[1]重庆大学电气工程学院高电压与电工新技术教育部重点实验室,重庆400044 [2]重庆医科大学附属第二医院,重庆400010

出　　处：《生物医学工程学杂志》2008年第5期1206-1209,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金重点项目资助(50637020)

摘　　要：近年来,国内外学者对纳秒脉冲电场应用于细胞内电处理效应进行了一些较为深入的实验和机制研究,实验结果表明纳秒脉冲电场不会导致细胞膜电穿孔,但能够诱导凋亡、钙离子释放、基因表达增强、DNA和染色体破碎等细胞内电处理效应。为了解释细胞内电处理,研究者们认为当脉冲电场的脉宽大于细胞膜充电时间常数时,脉冲电场主要作用在细胞外膜上产生电穿孔。随脉冲宽度变小,电场对细胞核及核膜影响增强,当脉宽小于细胞膜充电时间常数时,脉冲电场主要作用于膜内细胞器,进行细胞内电处理。In recent years, many experts have done some researches on experiment and mechanism of intracellular electromanipulation （IEM） under nanosecond pulsed electric field （nsPEF）. The experiment results have shown that nsPEF could not induce electroporation of cell membrane, but could induce intracellular effects such as apoptosis, calciurn release, enhancement of gene expression, and fragmentation of DNA and chromosome. In order to account for the phenomenon, researchers believe that when the pulse width of the pulsed electric field is larger than the charging time of plasma membrane, the pulsed electric field mainly targets on the outer membrane of cell; and that the effect of the pulsed electric field on nucleus and nuclear membrane increases with the decrease of the pulse width. It is also believed that the effect of electroporation changes from the outer membrane to intracellular electromanipulation when the pulse width decreases to a value being smaller than the charging time of plasma membrane.

关 键 词：纳秒脉冲电场 细胞内电处理 跨膜电位 凋亡 

分 类 号：Q27[生物学—细胞生物学]