麻疹-流行性乙型脑炎联合减毒活疫苗研制的初步结果  

The Primary Development Result of Measles and Japanese Encephalitis Combined Attenuated Live Vaccine

作　　者：李萍萍[1] 魏树源[1] 孙凌聪[1] 易玲[1] 桂金拄[1] 吕文利[1] 刘晓娜[1] 夏莉莉[1] 胡德祥[1] 胡志雄[1] 程容华[1] 杨邦玲[1] 田华[1] 邱雁[1] 付格[1] 王新华[1] 杨艳珠[1] 

机构地区：[1]武汉生物制品研究所,湖北武汉430060

出　　处：《中国疫苗和免疫》2008年第5期385-389,共5页Chinese Journal of Vaccines and Immunization

基　　金：国家863课题基金资助(编号2006AA02A233)

摘　　要：目的研制麻疹-流行性乙型脑炎(乙脑)联合减毒活疫苗(Measles and Japanese Encephalitis Combined At-tenuated Live Vaccine,M-JEV)。方法选用我国自主开发的麻疹病毒沪-191疫苗株和乙脑病毒SA14-14-2疫苗株,制备单价麻疹减毒活疫苗(Measles Attenuated Live Vaccine,MV)和乙脑减毒活疫苗(Japanese Encephalitis At-tenuated Live Vaccine,JEV)原液,进行干扰试验、配比试验、保护剂筛选试验、稀释液筛选试验、冻干曲线的选择。结果确定了M-JEV的制备工艺,进行了M-JEV的中试。中试M-JEV经全面检定符合2005年版《中华人民共和国药典》三部单价MV和JEV质量标准。动物试验未见病毒间交叉干扰,未见加重反应,未影响小鼠的免疫应答,表现了良好的安全性和免疫原性。结论成功制备了M-JEV。Objective To develop measles and Japanese encephalitis（JE）combined attenuated live vaccine. Methods The independent development of China′s Shanghai 191 measles vaccine strains and Japanese encephalitis SA14-14-2 vaccine strains were selected to prepare two of monovalent vaccines solution,which were used to conduct the interference test,the ratio test,the screening test of protection agent,the screening test of dilution,and the alternative of the curve of freeze-dried.Results The preparation technology of measles and Japanese encephalitis combined live attenuated vaccine was determined.The batches of the products met the quality requirement of the three kinds of monovalent vaccine in the Pharmacopoeia of the people＇s Republic of China（edition 2005,volume 3）.The cross-interference and increased response were not found in the animal test,and the immune response of mouse was not influenced,which indicated the safety and immunogenicity of with good combined vaccine.Conclusion Measles and Japanese encephalitis combined attenuated live vaccine was developed successfully.

关 键 词：麻疹-流行性乙型脑炎联合减毒活疫苗 安全性 免疫原性 

分 类 号：R186[医药卫生—流行病学] R511.1[医药卫生—公共卫生与预防医学]