益肾活血胶囊对兔血管平滑肌细胞TGF-β1表达的影响  

The effect of capsule of tonifying kidney activating blood circulation on TGF-β1 expression of vascular smooth muscle cell in the rabbits

作　　者：来丽萍[1] 张继东[1] 刘粉叶[1] 于淑东[2] 

机构地区：[1]山东大学齐鲁医院中医科,山东济南250012 [2]山东省千佛山医院耳鼻喉科

出　　处：《中国老年学杂志》2008年第19期1873-1875,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(30572452)

摘　　要：目的探讨益肾活血胶囊提取液(YSHXC)对同型半胱氨酸(HCY)诱导的兔血管平滑肌细胞(VSMCs)转化生长因子β1(TGF-β1)表达的影响。方法组织贴块法体外原代培养兔VSMCs,由HCY诱导建立细胞增殖模型并分组:正常组、HCY刺激组(HCY1mmol/L)、HCY+叶酸组(HCY1mmol/L+叶酸5mmol/L)、HCY+YSHXC大剂量组(HCY1mmol/L+YSHXC5000mg/L)、HCY+YSHXC小剂量组(HCY1mmol/L+YSHXC500mg/L)。分别采用免疫组化及RT-PCR技术检测各组TGF-β1蛋白及mRNA的表达,同时通过MTT比色法测定细胞增殖活度。结果HCY刺激后细胞数目明显增多,细胞内TGF-β1表达显著增强(P<0·01),而YSHXC大、小剂量组细胞增殖活度减低,TGF-β1蛋白和mRNA的表达水平均下降(P<0·01),并呈现剂量依赖性(P<0·05),且此抑制作用优于叶酸组(P<0·01或P<0·05)。结论YSHXC可明显抑制HCY诱导的细胞内TGF-β1的表达,减少平滑肌细胞的增殖,这可能对延缓动脉粥样硬化进程起到一定的作用。Objective To explore the effect of extracting solution of capsule of tonifying kidney activating blood circulation （YSHXC） on TGF-β1 expression of vascular smooth muscle cells （VSMCs） in the rabbits induced by homocysteine （HCY）. Methods Primary VSMCs cultured by attachment-block in vitro were induced by HCY to establish cell multiplication model and divide into normal, HCY stimulation （HCY 1 mmol/L）, HCY ＋ folic acid （HCY 1 mmol/L ＋ folic acid 5 mmol/L）, HCY ＋ high dosage of YSHXC （HCY 1 mmol/L ＋ YSHXC 5 000 mg/L） ,HCY ＋ low dosage of YSHXC （HCY 1 mmol/L ＋ YSHXC 500 mg/L） groups. TGF-β1 protein and mRNA expressions were respectively detected by immunohistochemisty and RT-PCR. Activity of cell multiplication was determined by MTr colorimetric technique. Results After HCY stimulation, cell number obviously increased, TGF-β1 expression significantly enhanced （ P 〈 0. 01 ）, but high and low dosage of YSHXC groups had the decreased activity of cell multiplication ,the descended TGFβ1 protein and mRNA expressions （ P 〈 0. 01 ）, presenting dose dependent （ P 〈 0. 05 ）, the better effect than folic acid group （ P 〈 0. 01 orP 〈 0. 05 ）. Conclusions YSHXC could obviously inhibit TGF-β1 expressions induced by HCY,decrease VSMCs proliferation,which might be play a certain role in delaying atherosclerosis process.

关 键 词：益肾活血胶囊 转化生长因子Β1 血管平滑肌细胞 增殖 

分 类 号：R543.1[医药卫生—心血管疾病]