HPLC法测定千里香叶中黄酮类成分的含量  

HPLC determination of flavonoids in leaves of Murraya paniculata L.Jack

作　　者：闫江红[1] 马彦冬[2] 王晓中[2] 杨瑞杰[2] 李绪文[2] 马双成[3] 金永日[2] 

机构地区：[1]吉林省中医药科学院,长春130021 [2]吉林大学化学学院,长春130012 [3]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2008年第10期1630-1632,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:对千里香叶中的2种主要黄酮类成分5,7,3′,4′-四甲氧基黄酮(化合物I)和5,7,3′,4′,5′-五甲氧基黄酮(化合物Ⅱ)进行含量测定。方法:采用反相高效液相色谱法,使用 ZORBAX Extend C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(58:42),流速1.2 mL·min^(-1),检测波长337 nm,柱温25℃。结果:化合物Ⅰ、Ⅱ进样量在0.5～5μg范围内均有良好的线性关系,相关系数 r 均为0.9999(n=6);化合物Ⅰ、Ⅱ的平均回收率(n=6)分别为102.8%和99.4%,RSD 分别为1.0%和1.4%。结论:本方法能够准确地测定千里香叶中的2种主要黄酮类化合物的含量,测定方法简便、快捷。Objective：To determine two flavonoids——5,7,3＇ ,4＇ -tetramethoxyflavone（ compound Ⅰ ） and 5,7, 3＇ ,4＇, 5＇ - pentamethoxyflavone （ compound Ⅱ ） in leaves of Murraya paniculata L. Jack. Methods： Using RP - HPLC. The separation was performed on ZORBAX Extend C18 column（250 mm ×4. 6 mm,5μm） ,using a mixture of 58% methanol and 42% water as the mobile phase with the flow rate of 1.2 mL · min^ -1 at 25 ℃ ,the wavelength for measurement was 337 nm. Results：The linearity was in the range of 0.5 -5 μg（ n = 6） for compounds Ⅰ and Ⅱ , and all the correlation coefficients were 0. 9999 ; The average recoveries （ n = 6） for compounds Ⅰ and Ⅱ were 102. 8% （ RSD = 1.0% ） and 99.4% （ RSD = 1.4% ） respectively. Conclusion： This method is accurate, reliable and reproducible to determine two flavonoids in leaves of Murraya paniculata L. Jack

关 键 词：千里香 黄酮 高效液相色谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]