应用中空纤维法评价酪丝缬肽对肝癌的抑制作用  

Evaluation of the inhibitory effect of tripeptide tyroservatide on growth of human hepatocellular carcinoma using a hollow fiber assay

作　　者：石琳熙[1] 陆融[1] 王莉[1] 周春雷[1] 徐琼[1] 朱志峰[1] 马睿[1] 张华俐[1] 贾静[1] 傅正[1] 姚智[1] 

机构地区：[1]天津医科大学免疫学教研室,300070

出　　处：《中华肿瘤杂志》2008年第10期725-728,共4页Chinese Journal of Oncology

基　　金：国家高技术研究发展计划（863计划）资助项目（2004AA223170,2005AA223D40）;国家重大基础研究计划资助项目（2003CCA04300）;教育部科学技术研究重点资助项目（03007）

摘　　要：目的观察三肽化合物酪丝缬肽（YSV）的抗肝癌作用。方法应用新型四氮唑盐（MTS）法，检测YSV对BEL-7402、SMMC-7721、Hep3B、HepG2和SK-HEP-1人肝癌细胞体外生长的抑制作用；应用中空纤维管皮下移植模型，进一步观察YSV对这5种人肝癌细胞体内生长的影响。结果0．1～1．6mg／ml的YSV能够明显抑制BEL-7402、SMMC-7721、Hep3B、HepG2和SK—HEP-1人肝癌细胞的体外生长，YSV组的吸光度（A）值与相应阴性对照组相比，差异均有统计学意义（P〈0．05），其中YSV对人肝癌细胞BEL-7402的抑制作用最强，1．6mg／ml的YSV抑制率达63．3％。160μg·k^-1·d^-1和320μg·k^-1·d^-1的YSV均能够明显抑制BEL-7402、SMMC-7721、Hep3B、HepG2和SK—HEP-1人肝癌细胞的体内生长，YSV组的A值与相应生理盐水组相比，差异均有统计学意义（P〈0．05）。YSV对人肝癌细胞BEL-7402的抑制作用最强，320μg·k^-1·d^-1 YSV的抑制率为53．1％。结论中空纤维法可以快速、准确地观察药物对不同肝癌细胞株体内生长的抑制作用，为YSV的临床应用提供了实验指导和可靠证据。Objective To investigate the inhibitory effect of tyroservatide （YSV） on growth of hepatoeellular carcinoma cells. Methods In vitro effects of YSV on five human hepatocellular carcinoma cell lines were assayed by MTS. In vivo effects of YSV on 5 human hepatoeellular carcinoma cell lines were assayed by hollow fiber tumor model. Results After treatment with YSV at a dose of 0.1 - 1.6 mg/ml, the growth of the five cell lines was significantly inhibited in vitro compared with that of the control group （ P 〈 0.05 ）. Especially, YSL remarkably inhibited the growth of human hepatocellular carcinoma BEL-7402 cells, i.e. the cell growth was inhibited by 63.3% after treatment with YSL at 1.6 mg/ml. The hollow fiber tumor model demonstrated that YSL （ 320 μg·k^-1·d^-1 and 640 μg·k^-1·d^-1 ） treatment significantly inhibited the in vivo growth of the five cancer cell lines compared with that in the saline control （P 〈 0.05 ）. YSL showed the highest level of inhibition of human BEL-7402 hepatocellular carcinoma cells, with an inhibitory index of 53.1% at 320 μg·k^-1·d^-1. Conclusion As a new method, hollow fiber assay may be used to evaluate the inhibitory effect of drugs on different tumor cells in vivo, rapidly, accurately and economically. Our results provide an instruction and evidence for clinical use of YSV.

关 键 词：三肽化合物酪丝缬肽 肝细胞性肝癌 中空纤维法 

分 类 号：R686[医药卫生—骨科学]