三种化合物对温石棉生物学活性抑制作用的机制探讨  

Inhibitory mechanisms of three compounds for chrysotile-induced biological activities

作　　者：樊晶光[1] 王起恩[2] 

机构地区：[1]中国安全生产科学研究院,中国化学品安全协会,北京100029 [2]美国俄亥俄州立大学

出　　处：《中华劳动卫生职业病杂志》2008年第10期592-595,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的研究经用柠檬酸铝、混合稀土或亚硒酸钠溶液浸泡处理温石棉对温石棉表面元素含量的影响，探讨3种化合物对石棉生物学活性抑制作用的机制。方法用浓度分别为250、500、1000μg/ml的柠檬酸铝溶液，125、250、500、1000μg／ml的混合稀土溶液或亚硒酸钠溶液浸泡温石棉纤维10min或1h后，通过透射电镜，结合能谱分析，测定石棉纤维表面铝元素构成比；利用等离子发射光谱和质谱联用仪（ICP-MS）测定石棉纤维表面15种稀土元素的含量；利用荧光分光光度计间接测定石棉纤维表面硒元素含量。结果温石棉纤维对柠檬酸铝、混合稀土或亚硒酸钠均有吸附作用，经不同浓度柠檬酸铝、混合稀土或亚硒酸钠溶液浸泡处理10min或1h后，石棉纤维表面相应元素或离子的含量均随溶液浓度的增高而升高。结论用柠檬酸铝、混合稀土和亚硒酸钠溶液处理石棉，均可改变石棉纤维表面元素的构成和含量。上述3种化合物处理石棉后，可能通过“封闭”石棉表面某些“活性位点”，抑制石棉纤维的生物学活性。Objective To study the effects of aluminum citrate （AC）, rare earth compounds （ REC ） and sodium selenite （SS） on the surface elements of chrysotile fibers and the inhibitory mechanisms of three compounds for chrysotile-induced biological activities. Methods After being soaked in 250,500 and 1000 μg/ml aluminum citrate solutions, 125,250,500 and 1000 μg/ml mixed rare earths solutions or 125,250,500 and 1000 μg/ml sodium selenite solutions for 10 rain or 1 hour,the fabrication and the levels of surface elements of chrysotile fibers were determined. Results Aluminum citrate,mixed rare earths or sodium selenite all could be adsorbed by chrysotile fibers. After pretreatment of chrysotile fibers with aluminum citrate,mixed rare earths or sodium selenite solutions for 10 min or 1 hour, the corresponding elements or ion on the surface of chrysotile fibers increased with the increase of concentration of the solutions. Conclusion Pretreatment of chrysotile with aluminum citrate, mixed rare earths or sodium selenite solutions can change the fabrication and the levels of surface elements of chrysotile fibers, and inhibit the biological activities of chrysotile by ＂sealing＂ some active sites on the surface of chrysotile fibers.

关 键 词：石棉类 蛇纹石 元素 亚硒酸钠 金属 稀土 

分 类 号：R686[医药卫生—骨科学]