甜菜碱通过钙通道升高鼠脾淋巴细胞内[Ca^(2+)]i研究  

Study on investigation of calcium channel of betaine advancing mouse splen-lymphocytes [Ca^(2+)]i in vitro

作　　者：冯小燕[1,2] 高世勇[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2008年第5期513-517,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(30400352);教育部重点项目(205045);黑龙江省自然科学基金(D200611);黑龙江省研究生创新基金(YJSCX2006-0077HSD)

摘　　要：研究甜菜碱对小鼠脾淋巴细胞内钙离子浓度的变化及相关钙通道的研究.应用激光共聚焦显微镜(LSCM)测小鼠脾淋巴细胞内钙浓度的变化,应用不同钙通道阻滞剂研究甜菜碱影响细胞内钙浓度变化途径.对终浓度4 mmol/L甜菜碱作用淋巴细胞不同时间的细胞内钙离子浓度值分析表明:甜菜碱可以使淋巴细胞内Ca2+浓度升高,6 h后效果最明显;对加入不同阻滞剂细胞内钙离子浓度变化分析表明:钙通道及蛋白阻滞剂硝苯地平、地尔硫卓、咪贝地尔、金雀异黄素对甜菜碱升高淋巴细胞内钙离子浓度没有影响;维拉帕米、新霉素、肝素、普鲁卡因能阻断甜菜碱对淋巴细胞内钙离子浓度的升高作用.由此可知:细胞内钙离子浓度升高主要通过以下途径:在G蛋白介导下通过影响L-型电压门控钙通道的α1亚单位而引起外钙内流;通过影响胞内钙库的IP3R钙通道和RyR钙通道而引起内钙外排.其共同引起胞质钙离子浓度增加.To investigate the effects of Betaine on splen-lymphocytes intracellular Ca^2＋ concentration and correlated calcium channel in vitro. To observe the effects of the betaine on the [ Ca^2＋ ]i by LSCM, the path of Ca^2＋ entering into cell with different Ca^2＋ channel blocker. Analyzing the intracellular [ Ca^2＋ ] i, betaine can increasing the intracellular [ Ca^2＋ ] i, and the best times is 6 h ; Analyzing the intracellular [ Ca^2＋ ] i with Ca^2＋ channel blocker, the result is nifedipine, diltiazem, mibefradil, genistein can not affect the role of betaine increasing lymphocytes intracellular [ Ca^2＋]i and verapamil ,heparin, neomyci-sulfate ,procaine can block the role of betaine increasing lymphocytes intracellular [ Ca^2＋ ] i. Betaine can increasing intracellular [ Ca^2＋ ] i. and the way of increasing intracellular [ Ca^2＋ ] i as following ： It can influence the α1-subunit of L-VDC which is affected with G-protein; It can increasing intracellular [ Ca^2＋ ] i through influencing IP3R Ca^2＋ channel and RyR Ca^2＋ channel.

关 键 词：甜菜碱 细胞内CA^2+ 钙通道阻滞剂 

分 类 号：R258[医药卫生—中医内科学]