福州市乙肝病毒基因型和基因亚型的分布及其与前C、C基因启动子变异的关系  

Distribution of Hepatitis B virus genotypes/subgenotypes and the relationship between genotypes and precore/core promotor mutaions in Fuzhou,China

作　　者：郭燕[1] 郑能雄[1] 姚栩[1] 纪惠玲[1] 陈智伟[1] 陈艳[1] 徐珊[1] 张彩云[1] 黄晓霞[1] 杨建娜[1] 卢园[1] 

机构地区：[1]福州市疾病预防控制中心,福建福州350004

出　　处：《中国病原生物学杂志》2008年第10期731-734,共4页Journal of Pathogen Biology

基　　金：福州市科委资助项目(No.2003-27)

摘　　要：目的研究福州市乙肝病毒基因型和亚型分布及其与T1762/A1764、A1896变异的关系,为完善预防、诊断、治疗病毒感染的策略和方法提供科学依据。方法应用型特异性引物PCR法检测HBsAg阳性血清的基因型,应用PCR-RFLP方法检测基因亚型、T1762/A1764变异和A1896变异。结果282份HBsAg阳性血清样品中103份未能成功分型,其余179份样品中B基因型122份(68.2%),C基因型54份(30.2%),B+C型3份(1.7%),未检测到其他基因型。随机选取的100份B基因型样品中,Ba亚型71份(71.0%),Bj亚型8份(8.0%),未能分亚型者21份(21.0%)。54份C基因型样品中Ce亚型31份(57.4%),Cs亚型14份(25.9%),Ce+Cs1份(1.9%),未能分亚型者8份(14.8%)。T1762/A1764变异标本9份(8.7%),Ce亚型变异率最高(29.2%),Ba亚型次之(3.3%),Cs和Bj亚型未检测到变异株,T1762/A1764变异在不同基因型和亚型间的分布差异有统计学意义(P<0.05)。A1896变异标本10份(10.0%),Ba亚型变异率最高(14.0%),Cs亚型次之(10.0%),Ce亚型最低(4.0%),不同基因型和亚型中的变异差异无统计学意义(P>0.05)。HBeAg阳性和阴性样品中的基因型和亚型分布差异无统计学意义(P>0.05)。结论福州市乙肝病毒以B、C基因型为主,Ba、Ce亚型占优势,HBV各基因型和亚型发生T1762/A1764、A1896变异的模式不同。Objective To investigate the distribution of HBV genotypes/subgenotypes and the relationship between genotypes and basic core promoter （T1762/A1764） and precore stop code （A1896） mutations in Fuzhou. Methods HBV genotypes were detected by PCR using type-specific primers, the subgenotypes, T1762/A1764 and A1896 mutations were detected by PCR RFLP. Results In 282 specimens, 103 could not be identified successfully, 122 were genotype B （68.2%）, 54 weregenotypeC （30.2%） and 3 weregenotypeB＋C （1.7%）. In the 100 specimen selected fromgenotype B randomly, 71 were subgenotype Ba （71.0%）, 8 were subgenotype Bj （8.0%） and 21 （21.0 %） could not be identified. In genotype C, 31 were Ce （57.4%）, 14 were Cs （25.9%）, 1 was Ce＋Cs （1.9%） and 8 （14.8%） could not be identified. Prevalence of TI762/A1764 was higher inCe （29.2%） thanBa （3.3%）, significant difference was found compared with different genotypes and subgenotypes （P〈 0. 05）. Development of A1896 was higher in Ba （14. 0%） than Cs （10.0%） and Ce （4.0%）, but there was no significant difference among them （P〉0.05）. Besides, there was no significant difference in specimen with positive or negative HBeAg （P〉0.05）. Conclusion Genotype B and C, especially Ba and Ce, were predominant in Fuzhou and the incidence of T1762/A1764 and A1896 mutations is different from HBV genotypes and subgenotypes.

关 键 词：肝炎病毒 乙型 基因型 基因亚型 T1762/A1764变异 A1896变异 

分 类 号：R373.21[医药卫生—病原生物学]