老年人腹主动脉瘤血管平滑肌细胞相关蛋白表达及巨噬细胞浸润的病理学特点  

Expressions of associated proteins in vascular smooth muscle cells and infiltration of macrophages in elderly abdominal aortic aneurysms

作　　者：庄世虹[1] 周洪莲[1] 黄敏[1] 聂斌[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院老年病科,武汉市430030

出　　处：《中华老年医学杂志》2008年第10期750-752,共3页Chinese Journal of Geriatrics

摘　　要：目的观察血管平滑肌细胞（VSMC）表达变化及巨噬细胞浸润在老年人腹主动脉瘤中的病理学特点。方法对15例老年人腹主动脉瘤与6例正常腹主动脉组织行HE染色、Van Gieson法染色和免疫组织化学染色。用免疫组织化学染色检测α-平滑肌肌动蛋白（α-SMA）、组织蛋白酶B及CD68蛋白表达。结果老年人腹主动脉瘤病变处胶原容积百分比（9．3±1．9）％，较正常主动脉的（5．3±1．8）％增高（P〈0．05）。老年人腹主动脉瘤中组织蛋白酶B和CD68的表达增强分别为0．38±0．07和0．51±0．12，α-SMA表达减弱为0．23±0．05，与正常腹主动脉（分别为0．13±0．06和0．01±0．01，0．33±0．05）比较，差异有统计学意义（P〈0．05）。结论VSMC相关蛋白表达水平改变及巨噬细胞浸润可能参与了老年人腹主动脉瘤血管壁的破坏。Objective To investigate the pathological characteristics of the expressions of associated proteins in vascular smooth muscle cells and infiltration of macrophages in elderly abdominal aortic aneurysms. Methods HE stained slices, Van Gieson＇ s stained slices and immunohistochemical staining were applied to detect protein expression in the tissue sections of 15 cases of elderly abdominal aortic aneurysms and 6 cases of normal abdominal aorta. The protein expressions of α-smooth muscle actin, cathepsin B and CD68 were detected by immunohistochemical staining. Results Compared with normal abdominal aorta, the collagen content was higher in elderly abdominal aortic aneurysms [（9.3 ± 1.9） % vs. （5.3 ± 1.8） %, P〈0. 05]. The levels of expression of cathepsin B and CD68 were higher in elderly abdominal aortic aneurysms than those in normal abdominal aorta , but the level of expression of α-SMA was lower in elderly abdominal aortic aneurysms than that in normal abdominal aorta[（0. 38±0. 07） vs. （0.13±0.06）, （0.51±0.12） vs. （0. 01±0. 01）, （0.23±0.05） vs. （0.33±0.05） ,respectively, all P〈0. 053. Conclusions Changes in the expression of associated proteins in vascular smooth muscle cells and infiltration of macrophages may participate in the vascular walls destruction in elderly abdominal aortic aneurysms.

关 键 词：主动脉 腹 组织蛋白酶B 肌 平滑 血管 

分 类 号：R686[医药卫生—骨科学]