Kinetic properties of nicotinic receptors in cultured rat sympathetic neurons from superior cervical ganglia  

作　　者：郑建全[1] 何湘平[1] 杨爱珍[1] 刘传缋[1] 

机构地区：[1]军事医学科学院毒物药物研究所

出　　处：《中国药理学报》1997年第4期330-333,共4页Acta Pharmacologica Sinica

摘　　要：目的：研究培养的新生大鼠颈上神经节交感神经元烟碱受体的动力学特性．方法：膜片箝技术的全细胞记录方法，记录不同浓度烟碱诱发的电流，使用Ｃｌａｒｋ方程对烟碱作用的量效曲线进行拟合．结果：１０，２０，４０，８０和１６０μｍｏｌ·Ｌ－１烟碱诱发电流的幅度分别为：０９１±００８，１５６±０１４，２５３±０２７，３９３±０４６和４５７±０５５ｎＡ（ｎ＝１５），经Ｃｌａｒｋ方程拟合，得到Ｈ＝１０９７，Ｅｍａｘ＝５９５８ｎＡ，Ｋ＝７３０６１μｍｏｌ·Ｌ－１，将Ｈ＝１时拟合得到的Ｅｍａｘ（６５１３ｎＡ）和Ｋ值（６１４５７μｍｏｌ·Ｌ－１）代入Ｃｌａｒｋ方程，所计算出的理论值与相应浓度烟碱诱发电流的实测值基本相符．结论：烟碱与交感神经元烟碱受体作用的动力学特性符合一个作用位点的反应模型．IM: To analyze the kinetic properties of the effect of nicotine on nicotinic acetylcholine receptors (nAChR) in the cultured sympathetic neurons from neonatal rat superior cervical ganglia (SCG). METHODS: The whole cell recording method of patch clamp technique was used to record the currents induced by different concentrations of nicotine. The concentration response of nicotine was fitted with Clark equation. RESULTS: Hill coefficient (1 097) was determined by fitting the nicotine responses of neuronal nAChR with Clark equation. The theoretical values of nicotine effect, calculated with Clark equation with H =1, were basically identical with the practically recorded currents. CONCLUSIONS: Interaction of nicotine and nAChR in rat SCG fits a single binding site model.

关 键 词：烟碱 烟碱受体 交感神经节 药物动力学 

分 类 号：R971.9[医药卫生—药品] R969.1[医药卫生—药学]