Effect of 3,6-dimethamidodibenzopyriodonium citrate on Ca^(2+) in rabbit platelet in vitro  

作　　者：魏宝贤[1] 袁素芬[1] 邱培伦[1] 尹钟洙[2] 

机构地区：[1]西安医科大学生化教研室 [2]中日友好医院临床医学研究所药理室

出　　处：《中国药理学报》1997年第4期357-358,共2页Acta Pharmacologica Sinica

摘　　要：目的：观察Ｉ６５对家兔血小板胞浆游离钙离子（［Ｃａ２＋］ｉ）浓度的影响．方法：用Ｑｕｉｎ２ＡＭ荧光探针在体外测定家兔血小板［Ｃａ２＋］ｉ．结果：ＣａＣｌ２１ｍｍｏｌ·Ｌ－１时，Ｉ６５（１０，２０和３０μｍｏｌ·Ｌ－１）使血小板［Ｃａ２＋］ｉ分别由１４２±２２ｎｍｏｌ·Ｌ－１和１２４±１８ｎｍｏｌ·Ｌ－１减少到１１８±２０，７８±１２，４０±１０ｎｍｏｌ·Ｌ－１和１０８±１５，７７±１４，３７±１４ｎｍｏｌ·Ｌ－１．用依他酸２ｍｍｏｌ·Ｌ－１络合胞外Ｃａ２＋，Ｉ６５胞内贮存Ｃａ２＋释放从５２±１１ｎｍｏｌ·Ｌ－１减少至３４±９，１９±６和１１±５ｎｍｏｌ·Ｌ－１．另外，Ｉ６５也使胞外Ｃａ２＋跨膜内流从９１±１３ｎｍｏｌ·Ｌ－１分别降至８４±１５，５８±１５和２８±１９ｎｍｏｌ·Ｌ－１．结论：Ｉ６５不仅明显抑制家兔血小板胞外Ｃａ２＋跨膜内流而且抑制胞内贮存Ｃａ２＋释放．IM: To study the effect of 3,6- dimethamidodibenzopyriodonium citrate (I 65) on the cytoplasmic free Ca^2+ ([Ca^2+ ] i) concentration in rabbit platelet. METHODS: Measurement of the cytosolic Ca^2+ of platelets in vitro by using Quin 2 AM fluorescence technique. RESULTS: In the presence of CaCl 2 1 mmol·L -1 , I 65 (10, 20, and 30 μmol·L -1 ) reduced the rise in [Ca^2+ ] i induced by thrombin and calcimycin from 142±22 nmol ·L -1 and 124±18 nmol·L -1 to 118±20, 78±12, 40±10 nmol·L -1 , respectively and 108±15, 77 ±14 , 37±14 nmol·L -1 , respectively. In the presence of egtazic acid 2 mmol·L -1 , I 65 (10, 20, and 30 μmol·L -1 ), reduced the Ca 2+ release induced by thrombin from 52±11 nmol·L -1 to 34±9, 19±6, and 11±5 nmol·L -1 , respectively. In addition, I 65 (10, 20, and 30 μmol·L -1 ) also reduced the Ca 2+ influx induced by thrombin from 91±13 nmol·L -1 to 84±15, 58±15, and 28±19 nmol·L -1 , respectively. CONCLUSION: I 65 inhibited not only the Ca 2+ release, but also the influx of Ca 2+ in activation platelet.

关 键 词：碘Wong化合物 凝血酶 钙 血小板 维拉帕米 

分 类 号：R973.2[医药卫生—药品]