甘蓝型油菜异胡豆苷合成酶(Strictosidine synthase)cDNA的克隆与分析  

Cloning and sequence analysis of Strictosidine synthase cDNA in Brassica napus

作　　者：王敏培[1] 周云涛[1] 王茂林[1] 赵云[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2008年第5期1277-1280,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金项目(30571174)

摘　　要：以无花瓣与正常甘蓝型油菜幼嫩花蕾(长度小于0.2mm)建立的差异表达文库中发现的异胡豆苷合成酶基因(STR)的一个cDNA片段为基础,利用RACE方法获得了甘蓝型油菜的STR基因cDNA全序列BnSTR.序列分析表明BnSTR全长1408bp,开放阅读框从第20个碱基到第1291个碱基,推导的蛋白序列与Arabidopsis thaliana,Lycopersicon esculentum,Oryzasativa,Rauvolfia manni,Catharanthus roseus和Ophiorrhiza pumila中STR的同源性分别是91,41.8,36.2,31.2,29.2和28.4%.表达结果显示,与野生型相比,油菜BnSTR在无花瓣突变型幼嫩花蕾中表达量明显下降,BnSTR可能与花瓣的发育有关.A partial STR-like cDNA section sequence in Brassia napus was discovered in a suppression subtractive library constructed with early flower buds （length less than 0.2 mm） of an apetalous line and its wild type. According to the partially known sequence, the gene-specific primers were designed to amplify the 5′ and 3′ cDNA ends, respectively. The full-length cDNA of this STR-like gene, named BnSTR, was cloned through RACE approach. Sequence analysis showed that BnS＇TR was 1408 bp long and contained an ORF from the 20th bp to the 1291th bp. BnSTR protein sequence was 91, 41.8, 36.2, 31.2,29.2 and 28.4% identical to STR-like family members in Arabidopsis thaliana, Lycopersi con esculentum, Oryza sativa, Rauvol fia rnanni, Catharanthus roseus and Ophiorrhiza pumila respectively. Expression analysis showed that compared with that in wild type, the expression of BnSTR obviously declined in early flower buds in the apetalous line Apet33-10, revealing that the function of BnSTR could be associated with petal primordial initiation and growth.

关 键 词：异胡豆苷合成酶 无花瓣突变体 RACE—PCR 甘蓝型油菜 

分 类 号：Q78[生物学—分子生物学]