用细胞钓蛋白带技术初探脊髓源性神经营养活性物质  

PRELIMINARY EXPLORATION OF SPINAL DERIVED NEUROTROPHIC SUBSTANCE USING PROTEIN BAND FISHING BY CELL TECHNIQUE

作　　者：王廷华[1] 吴良芳[1] 廖德阳 

机构地区：[1]华西医科大学组织学研究室,成都610041

出　　处：《神经解剖学杂志》1997年第4期339-342,共4页Chinese Journal of Neuroanatomy

摘　　要：用5只单侧后肢备用根猫术后5d的两侧脊髓背角组织提取液进行聚丙烯酰肢凝胶电泳，再以鸡胚背根节种经细胞构蛋白带技术找寻有神经营养活性的蛋白带．结果显示：手术侧组的电泳凝胶条上相对迁移享0．10～0．13和0．43～0．50两处均有较多的神经细胞附着，细胞数分别是165个2.5×105μm2、216个／2．5×105μm2．非手术侧组凝胶条相应两处同一面积内仅有1或2个细胞．两侧相比有显著性差异（P＜0001），提示手术侧组电泳凝胶条相对迁移率0．10～013与043～0．50两处含有能维持种经元存活的神经营养活性物质．Our previous studies demonstrated that the extract from the spinal dorsal horn of unilateral L1～S2 partial dorsalrhizotomy cats promoted obviously neurite-outgrowth in vitro. In order to find the neurotrophic substance in dorsal horn ofspared root animal, five cats with unilateral spared dorsal root of hind limb (L1～S2 dorsal roots sectioned, except L6 asspared root) were used. Five days after operation, the dorsal horn of both sides from T12～S3 spinal cord were taken respec-tively. After homogenization and centrifugalization, the supernatant was taken and employed for polyacrylamide gel elec-trophoresis (PAGE). A simple protein band fishing by cells (PBFC) method was used to find out the protein band havingneurotrophic activity. The results were as following: There were consistently many survival neurons in mobility relativefront (Rf) 0. l0~ 0. 13 (165 cells/2. 5 X l05 pm' ) and 0. 43~ O. 50(216 cells/2. 5 X 1Os pm')0n 0perated side group gel after 48h culture. H0wever, there were almost no survivaI neurons (l^2 '..ll./2. 5 X 1O5 pmz) in the same area on unoperated sidegroup geI (Pr0. O01). The resuIt revealed that there was neurotrophic substance f0r maintaining neuron survivaI in Rf 0. 0l^0. 13 and 0. 43~0. 5O bands of operated side group gel.

关 键 词：神经营养 活性物质 脊髓背角 细胞钓蛋白带 

分 类 号：Q593.203[生物学—生物化学]