药物头孢Ⅳ号和氨苄青霉素及其生物合成前体的毛细管区带电泳分离  

Separation of Drugs Cephalexin, Ampicillin, and Their Biosynthetic Precursors L Phe L Cys D Val and D Phe L Cys D Val by Capillary Zone Electrophoresis

作　　者：王志[1] 孙亦梁[1] 王丹丹 

机构地区：[1]北京大学化学与分子工程学院

出　　处：《色谱》1997年第5期378-380,共3页Chinese Journal of Chromatography

基　　金：国家教委博士点基金;国家自然科学基金

摘　　要：研究了同分异构体药物头孢Ⅳ号和氨苄青霉素及其目标生物合成前体三肽Ｄ－苯丙氨酸－Ｌ－半胱氨酸－Ｄ－缬氨酸、Ｌ－苯丙氨酸－Ｌ－半胱氨酸－Ｄ－缬氨酸的毛细管区带电泳分离。结果表明，采用含１２ｍｍｏｌ／Ｌ二甲基β－环糊精（ＤＭ－β－ＣＤ）的５０ｍｍｏｌ／ＬＴｒｉｓ－Ｈ３ＰＯ４缓冲溶液（ｐＨ２．３）既可将头孢Ⅳ号和氨苄青霉素分离，又可将Ｄ－苯丙氨酸－Ｌ－半胱氨酸－Ｄ－缬氨酸、Ｌ－苯丙氨酸－Ｌ－半胱氨酸－Ｄ－缬氨酸分离。The separation of drugs cephalexin, ampicillin and their biosynthetic precursors L Phe L Cys D Val and D Phe L Cys D Val was investigated by using capillary zone electrophoresis in acidic Tris H 3PO 4 buffer at pH 2.3. With 50mmol/L Tris H 3PO 4 (pH 2.3) buffer, L Phe L Cys D Val and D Phe L Cys D Val were only partially separated whereas near baseline separation was achieved between cephalexin and ampicillin. The experiments showed that the addition of β type cyclodextrins (β CD) to the running buffer can influence the separation selectivity. When β CD was used as buffer additives at 12mmol/L, the separation between L Phe L Cys D Val and D Phe L Cys D Val was much improved, however, the separation between cephalexin and ampicillin was impaired. When using 50mmol/L Tris H 3PO 4 containing 12mmol/L DM β CD (pH 2.3) buffer system, all compounds investigated were completely separated. This separation method is simple, fast and casy to operate. It involves only the conventional reagents with a much lower run cost than using HPLC. It is expected to be used in the synthetic process monitoring.

关 键 词：毛细管区带电泳 头孢Ⅳ号 氨苄青霉素 前体 

分 类 号：O657.8[理学—分析化学] TQ465.1[理学—化学]